Summary
Ouabain inhibitable active Rb+ uptake was compared in canine Purkinje fibre and the left ventricular muscle to define the possible difference in electrophysiological sensitivity to ouabain in the two tissues. Ouabain inhibitable, active uptake of Rb+ was calculated from the difference between total Rb+ uptake assayed in the absence of ouabain and nonspecific Rb+ accumulation assayed in the presence of 10−4 mol/l ouabain. A similar degree of active Rb+ uptake was inhibited by ouabain in both Purkinje fibre and the left ventricular muscle from the same animal, even though the Purkinje fibre took up more 86Rb+ than the ventricle. The specific tissue binding of ouabain in vitro was also not significantly different in the functionally different two tissues. It is concluded that there is no differential ouabain sensitivity of Rb+ uptake, in canine Purkinje fibre and ventricular muscle.
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References
Bernstein JC, Israel Y (1970) Active transport of 86Rb+ in human red cells and rat brain slices. J Pharmacol Exp Ther 174:323–329
Cranefield PF (1975) The conduction of the cardiac impulse. Futura Publishing Co., Mount Kisco, New York, pp 75–113
Forbush, B, Hoffman JF (1979) Evidence that ouabain binds to the same large peptide chain of dimeric Na+, K+-ATPase that is phosphorylated from Pi. Biochemistry 18:2308–2315
Glynn IM (1957) The action of cardiac glycosides on sodium and potassium movements in human red cells. J Physiol 136:148–173
Hilden S, Rhee HM, Hokin LE (1974) Sodium transport by phospholipid vesicles containing purified Na, K-ATPase. J Biol Chem 249:7432–7440
Hollander PB (1975) Differential sensitivities of Purkinje fibers and ventricular myocardium to ouabain toxicities. J Clin Pharmacol 15:560
Hougen TJ, Smith TW (1978) Inhibitions of myocardial monovalent cation active transport by subtoxic doses of ouabain in the dog. Circ Res 42(6):856–863
Ku D, Akera T, Pew CL, Brody TM (1974) Cardiac glycosides: Correlations among Na+, K+-ATPase, sodium pump and contractility in the guinea pig heart. Naunyn-Schmiedebergs Arch Pharmak Exp Path 258:185–200
Kübler W, Smekal P von (1975) Comparative studies of the Na+, K+-ATPase activity and digitalis sensitivity in the nodal tissue and the myocardium. Acta Cardiol (Suppl) 17:103–113
Michael LH, Schwartz A, Wallick ET (1979) Nature of the transport adenosine triphosphatase-digitalis complex. XIV. Inotrophy and cardiac glycoside interaction with Na+, K+-ATPase of isolated cat papillary muscles. Mol Pharmacol 16:135–146
Moe GK, Mendez R (1951) The action of several cardiac glycosides on conduction of velocity and ventricular excitability in the dog heart. Circulation 4:729–734
Palfi FJ, Besch HR, Jr, Watanabe AM (1978) Ouabain sensitivity of the Na+, K+-ATPase activity from single bovine cardiac Purkinje fiber and adjacent papillary muscle. J Mol Cell Cardiol 10:1149–1155
Polimeni PI, Vassalle M (1970) Potassium fluxes in Purkinje and ventricle fibers during rest and activity. Am J Physiol 218(5):1381–1388
Polimeni PI, Vassalle M (1971) On the mechanism of ouabain toxicity in Purkinje and ventricular muscle fibers at rest and during activity. Am J Cardiol 27:622–629
Poole-Wilson PA, Cameron IR (1975) ECS, intracellular pH, and electrolytes of cardiac and skeletal muscle. Am J Physiol 229:1229–1304
Rhee HM, Hokin LE (1979) Inhibition of 3H-ouabain binding to purified Na+, K+-ATPase by antibodies against the catalytic subunit. Biochem Biophys Acta 558:108–112
Rhee HM, Dutta S, Marks BH (1976) Cardiac Na, K-ATPase activity during positive inotropic and toxic actions of ouabain. Eur J Pharmacol 37:141–153
Schwartz A, Lindenmayer GE, Allen JC (1975) The sodium-potassium adenosine triphosphatase: pharmacological, physiological, and biochemical aspects. Pharmacol Rev 27:1–134
Sherf L, James TN (1979) Fine structure of cells and their histologic organization within internodal pathways of the heart: clinical and electrocardiographic implications. Am J Cardiol 44:345–370
Skou J (1960) Further investigations of Mg+Na activated adenosine triphosphatase, possibly related to the active, linked transport of Na and K across the nerve membrane. Biochem Biophys Acta 42:6–23
Vassalle M Karis J, Hoffman BF (1962) Toxic effects of ouabain on Purkinje fibers and ventricular muscle fibers. Am J Physiol 203:433–439
Vick RL, Chang DC, Nichols BL, Hazelwood CF, Harvey MC (1973) Sodium potassium and water in cardiac tissues. Ann NY Acad Sci 204:575–606
Winegard S, Shanes AM (1962) Calcium flux and contractility of guinea pig atria. J Gen Physiol 45:371–394
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Rhee, H.M. Ouabain sensitivity of Rb+ uptake in canine Purkinje fibre and ventricular muscle. Naunyn-Schmiedeberg's Arch. Pharmacol. 318, 344–348 (1982). https://doi.org/10.1007/BF00501176
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DOI: https://doi.org/10.1007/BF00501176