Summary
Contradictory results on the role of NaK-ATPase as the diuretic receptor are reported in the literature. In own experiments the effects of 1 mM ouabain and 0.2 mM ethacrynic acid on fractional sodium reabsorption and oxygen consumption was investigated in an isolated perfused kidney system. NaK-ATPase was measured in basal-lateral membranes of kidney cortex tubular cells. Combination of ouabain and ethacrynic acid resulted in a complete inhibition of fractional sodium reabsorption at only a 46% inhibition of NaK-ATPase activity. The complete inhibition of sodium reabsorption at only a partial inhibition of NaK-ATPase probably is caused by additional metabolic effects of ethacrynic acid. It is suggested that NaK-ATPase is not the only diuretic receptor. — Another enzyme possibly involved in the control of sodium transport and water permeability is the renal adenyl cyclase. 1 mM furosemide and 1 mM ethacrynic acid inhibited the enzyme in homogenates of the kidney cortex and inner medulla, while amiloride was without any effect. In the cortex the furosemide induced inhibition of adenyl cyclase can be reversed by 1 U parathyroid hormone and 0.1 mM isoproterenol. The ethacrynic acid effect on adenyl cyclase was not influenced by isoproterenol. In the inner medulla 1 mU antidiuretic hormone but not isoproterenol antagonized the furosemide inhibition of adenyl cyclase. Antidiuretic hormone and isoproterenol exerted no effect on the ethacrynic acid inhibition of adenyl cyclase. It is suggested that furosemide and ethacrynic acid act at different sites of the adenyl cyclase or at a mixture of adenyl cyclases with different sensitivities.
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References
Agus, Z. S., Purchett, J. B., Senesky, D., Goldberg, M.: Mode of action of parathyroid hormone and cyclic adenosine 3′,5′-monophosphate on renal tubular phosphate reabsorption in the dog. J. clin. Invest. 50, 617–626 (1971)
Aulbert, E., Froese, P., Leipnitz, W., Ebel, H.: Preparation of basal-lateral membranes from rat kidney. Pflügers Arch. 339, R 50 (1973)
Beck, N. P., De Rubertis, F., Michelis, M. F., Fusco, R. D., Field, J. B., Davis, B. B.: Prostaglandin E1 inhibition of parathyroid hormone—induced increases in renal cortical adenyl cyclase activity, cyclic adenosine monophosphate concentration, 32P incorporation into phospholipid and in phosphate excretion, J. Lab. clin. Med. 76, 1005 (1970)
Beck, N. P., Kaneko, T., Zor, U., Field, J. B., Davis, B. B.: Effects of vasopressin and of prostaglandin E1 on the adenyl cyclase-cyclic 3′,5′-adenosine monophosphate system of the renal medulla of the rat. J. clin. Invest. 50, 2461–2465 (1971)
Beck, N. P., Reed, S. W., Murdaugh, H. V., Davis, B. B.: Effects of catecholamines and their interaction with other hormones on cyclic 3′,5′-adenosine monophosphate of the kidney. J. clin. Invest. 51, 939–944 (1972)
Bentley, P. J.: Amiloride: a potent inhibitor of sodium transport across the toad bladder. J. Physiol. (Lond.) 195, 317–330 (1968)
Bentley, P. J.: Actions of vasopressin and aldosterone on the toad bladder: inhibition by ethacrynic acid. J. Endocr. 43, 347–357 (1969)
Charnock, J. S., Almeida, A. F.: Ethacrynic acid accumulation by renal tissue. Biochem. Pharmacol. 21, 647–655 (1972)
Chignell, C. F., Titus, E.: Effect of adrenal steroids on a Na+ and K+-requiring adenosine triphosphatase from rat kidney. J. biol. Chem. 241, 5083–5089 (1966)
De Santo, N. G., Ebel, H., Hierholzer, K.: Plasma cell membranes of the rat kidney. II. ATPase activities in adrenalectomized rats with and without steroid hormone substitution. Pflügers Arch. 324, 26–42 (1971)
Duggan, D. E., Noll, R. M.: Effect of ethacrynic acid and cardiac glycosides upon a membrane adenosinetriphosphatase or renal cortex. Arch. Biochem. 109, 388–396 (1965)
Duggan, D. E., Noll, R.M.: A biochemical basis for the inactivity of ethacrynic acid in the rat. Biochem. biophys. Acta (Amst.) 121, 162–164 (1966)
Ebel, H., De Santo, N. G., Hierholzer, K.: Plasma cell membranes of the rat kidney. I. Purification and properties of cell membranes ATPase. Pflügers Arch. 324, 1–25 (1971)
Ebel, H., Ehrich, J., De Santo, N. G., Doerken, U.: Plasma membranes of the kidney. III. Influence of diuretics on ATPase activity. Pflügers Arch. 335, 224–234 (1972)
Ebel, H., Ozer, A., Sharp, G. W. G.: Renal adenyl cyclase as a diuretic receptor. 42th meeting (fall meeting), Deutsche Physiologische Gesellschaft, Hannover, October 1–5, 1973. Pflügers Arch. 343, Suppl., R47 (1973)
Ehrlich, E. N., Crabbé, J.: Amiloride and the mode of action of aldosterone on sodium transport across toad bladder and skin. Pflügers Arch. 204, 284–296 (1968)
Epstein, R. W.: The binding of ethacrynic acid to rabbit kidney cortex. Biochim. biophys. Acta (Amst.) 274, 119–127 (1972)
Ferguson, D. R.: Effects of frusemide on sodium and water transport by the isolated toad bladder. Brit. J. Pharmacol. 27, 528–531 (1966)
Fisher, D. A.: Norepinephrine inhibition of vasopressin antidiuresis. J. clin. Invest. 47, 540–547 (1968)
Fitzpatrick, D. F., Davenport, G. R., Forte, L., Landon, F. J.: Characterization of plasma membrane proteins in mammalian kidney. I. Preparation of a membrane fraction and separation of the protein. J. biol. Chem. 244, 3561–3569 (1969)
Gill, J. R., Casper, A. G.: Depression of proximal tubular sodium reabsorption in the dog in response to renal beta adrenergic stimulation by isoproterenol. J. clin. Invest. 50, 112–118 (1971)
Grantham, J. J., Burg, M. B.: Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules. Amer. J. Physiol. 211, 255–259 (1966)
Grantham, J. J., Orloff, J.: Effect of prostaglandin E1 on the permeability response of the isolated collecting tubule to vasopressin, adenosine 3′,5′-monophosphate, and theophylline. J. clin. Invest. 47, 115–1161 (1968)
Györy, A. Z., Brendel, U., Kinne, R.: Effect of cardiac glycosides and sodium ethacrynate on transepithelial sodium transport in in vivo micropuncture experiments and on isolated plasma membrane Na-K-ATPase in vitro of the rat. Pflügers Arch. 335, 287–296 (1972)
Handler, J. S., Butcher, R. W., Sutherland, E. W., Orloff, J.: The effect of vasopressin and of theophylline on the concentration of adenosine 3′,5′-phosphatate in the urinary bladder of the toad. J. biol. Chem. 240, 4524–4526 (1965)
Hendler, E. D., Torretti, J., Epstein, F. H.: The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney. J. clin. Invest. 50, 1329–1337 (1971)
Hook, J. B., Williamson, H. E.: Lack of correlation between natriuretic activity and inhibition of renal Na-K-activated ATPase. Proc. Soc. exp. Biol. (N.Y.) 120, 358–360 (1965)
Hynie, S., Sharp, G. W. G.: Adenyl cyclase in the toad bladder. Biochim. biophys. Acta (Amst.) 230, 40–51 (1971)
Jakobs, K. H., Schultz, K., Schultz, G.: Hemmung von Adenyl-Cyclase-Präparationen aus der Rattenniere durch Calciumionen und verschiedene Diuretika. Naunyn-Schmiedeberg's Arch. Pharmacol. 273, 248–266 (1972)
Jones, V. D., Lockett, G., Landon, F. J.: A cellular action of mercurial diuretics. J. Pharmacol. exp. Ther. 147, 23–31 (1965)
Jørgensen, P. L.: Regulation of the (Na++K+)-activated ATP hydrolyzing enzyme system in rat kidney. I. The effect of adrenalectomy and the supply of sodium on the enzyme system. Biochim. biophys. Acta (Amst.) 151, 212–224 (1968)
Katz, A. I., Epstein, F. H.: The role of sodium-potassium-activated adenosine triphosphatase in the reabsorption of sodium by the kidney. J. clin. Invest. 46, 1999–2011 (1969)
Kinne, R., Kinne-Saffran, E.: Isolierung und enzymatische Charakterisierung einer Bürstensaumfraktion der Rattenniere. Pflügers Arch. 308, 1–15 (1969)
Kinne, R., Schmitz, J. E., Kinne-Saffran, E.: The localization of the Na+−K+-ATPase in the cells of rat kidney cortex. A study on isolated plasma membranes. Pflügers Arch. 329, 191–206 (1971)
Landon, E. J., Fitzpatrick, D. F.: Symp. Proc. V th Int. Congr., Nephrol., Stockholm. Basel: Karger 1970
Lees, P., Lockett, M. F.: A study of the beta adrenergic receptors in rat kidney. Brit. J. Pharmacol. 20, 135–138 (1963)
Levi, J., Grinblat, J., Kleeman, C. R.: Effect of isoproterenol on water diuresis in rats with congenital diabetis insipidus. Amer. J. Physiol. 221, 1728–1732 (1971)
Liberman, B., Klein, L. A., Kleeman, C. R.: Effect of adrenergic blocking agents on the vasopressin inhibiting action of norepinephrine. Proc. Soc. exp. Biol. (N.Y.) 133, 131–134 (1970)
Martinez-Maldonado, M., Allen, J. C., Inageki, C., Tsaparas, N., Schwartz, A.: Renal sodium-potassium-activated adenosine triphosphatase and sodium reabsorption. J. clin. Invest. 51, 2544–2551 (1972)
Martinez-Maldonado, M., Eknoyan, G., Allen, J. C., Suki, W. N., Schwartz, A.: Urine dilution and concentration after digoxin infusion into renal artery of dogs. Proc. Soc. exp. Biol. (N.Y.) 134, 855–860 (1970)
Marumo, F., Edelman, I. S.: Effects of Ca++ and prostaglandin E1 on vasopressin activation of renal adenyl cyclase. J. clin. Invest. 50, 1613–1620 (1971)
Melson, G. L., Chase, L. R., Aurbach, G. D.: Parathyroid hormone-sensitive adenyl cyclase in isolated renal tubules. Endocrinology 86, 511–518 (1970)
Nechay, B. R., Nelson, J. A.: Renal ouabain-sensitive adenosine triphosphatase activity and Na+ reabsorption. J. Pharmacol. exp. Ther. 175, 717–726 (1970)
Nechay, B. R., Palmer, R. F., Chinoy, D. A., Posey, V. A.: The problem of Na+−K+ adenosine triphosphatase as the receptor for diuretic action of mercurials and ethacrynic acid. J. Pharmacol. exp. Ther. 157, 599–617 (1967)
Orloff, J., Handler, J. S.: The similarity of effects of vasopressin adenosine 3′,5′-phosphate (cyclic AMP), and theophylline on the toad bladder. J. clin. Invest. 41, 702–709 (1962)
Orloff, J., Handler, J. S.: The role of adenosine 3′,5′-phosphate in the action of antidiuretic hormone. Amer. J. Med. 42, 757–768 (1967)
Palmer, R. F., Nechay, B. R.: Biphasic renal effects of ouabain in the chicken: correlation with a microsomal Na+−K+ stimulated ATPase. J. Pharmacol. exp. Ther. 146, 92–98 (1964)
Pearson, J. E., Williams, R. L.: Analysis of direct renal actions of alpha and beta adrenergic stimulation upon sodium excretion compared to acetylcholine. Brit. J. Pharmacol. 33, 223–241 (1968)
Pitts, R. F.: Physiology of the kidney and body fluids: An introductory text, p. 97. Chicago: Year Book. Medical 1963
Proverbio, F., Robinson, J. W. L., Whitembury, G.: Sensitivities of (Na+−K+)-ATPase and Na+ extrusion mechanism to ouabain and ethacrynic acid in the cortex of the guinea pig kidney. Biochim. biophys. Acta (Amst.) 211, 327–336 (1970)
Radtke, H. W., Rumrich, G., Kinne-Saffran, E., Ullrich, K. J.: Dual action of acetazolamide and furosemide on proximal volume absorption in the rat kidney. Kidney Int. 1, 100–105 (1972)
Ross, B. D., Leaf, A., Epstein, F. H.: Evidence in the perfused kidney for Na reabsorption not mediated by Na-K-ATPase. V. Int. Congr. Nephrol, Mexico 1972, p. 138
Schmidt, U., Dubach, U. C.: Activity of (Na+K+)-stimulated adenosinetriphosphatase in the rat nephron. Pflügers Arch. 306, 219–226 (1969)
Schmidt, U., Dubach, U. C.: The behaviour of Na+K+-activated adenosine triphosphatase in various structures of the rat nephron after furosemide application. Nephron 7, 447–458 (1970)
Schrier, R. W., Liebermann, R., Uffermann, R. C.: Mechanism of antidiuretic effect of beta adrenergic stimulation. J. clin. Invest. 51, 97–111 (1972)
Schurek, H. J., Aulbert, E., Ebel, H.: Influence of ouabain and ethacrynic acid on sodium transport and NaK-ATPase activity in the isolated perfused rat kidney. Symposion on Biochemical aspects of renal function, Gdansk. Sept. 2–5, 1973
Taylor, C. B.: Effect of mercurial diuretics on adenosine-triphosphatase of rabbit kidney in vitro. Biochem. Pharmacol. 12, 539–550 (1963)
Toretti, J., Hendler, E., Weinstein, E., Longnecker, R. E., Epstein, F. H.: Functional significance of Na-K-ATPase in the kidney: effects of ouabain inhibition. Amer. J. Physiol. 222, 1398–1405 (1972)
Whittembury, G., Proverbio, F.: Two modes of Na extrusion from guinea pig kidney cortex slices. Pflügers Arch. 316, 1–25 (1970)
Windhager, E. E., Giebisch, G.: Electrophysiology of the nephron. Physiol. Rev. 45, 214–244 (1965)
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This work was supported by grants of the Deutsche Forschungsgemeinschaft.
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Ebel, H. Effect of diuretics on renal NaK-ATPase and adenyl cyclase. Naunyn-Schmiedeberg's Arch. Pharmacol. 281, 301–314 (1974). https://doi.org/10.1007/BF00500599
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DOI: https://doi.org/10.1007/BF00500599