Summary
A modification of the protein-binding assay for guanosine 3′,5′-monophosphate (Murad et al., 1971) is decribed yielding a substantial increase in sensitivity together with a more simplified procedure. The method employs a purified enzyme preparation from lobster tail muscle with a dissociation constant for cGMP of about 5 nM. The nucleotide-protein complex is separated from free cGMP by charcoal treatment. 0.05–0.1pmoles cGMP are detectable by this procedure. cGMP contents of seven discrete regions of rat brain were determined to show the usefullness of the described method.
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Dinnendahl, V. A rapid and simple procedure for the determination of guanosine 3′,5′-monophosphate by use of the protein-binding method. Naunyn-Schmiedeberg's Arch. Pharmacol. 284, 55–61 (1974). https://doi.org/10.1007/BF00499972
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DOI: https://doi.org/10.1007/BF00499972