Summary
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1.
The influence of oxyphenisatin (OP), a diphenolic laxative, and deoxycholate (DC) on the transfer of sodium and water in an everted sac preparation of stripped rat colon was investigated.
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2.
OP (10−5 M, mucosal side) and DC (3×10−4 M, mucosal side) completely blocked net water and sodium absorption. Net movements from the serosal to the mucosal side could not be induced by higher concentrations of the drugs.
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3.
Unidirectional sodium movements in both directions were increased by OP and DC.
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4.
The effect of DC on the sodium flux from the serosal to the mucosal side was reversible.
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5.
The potassium content of the mucosal epithelium was not changed by DC and OP.
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6.
The integrity of the epithelium, as judged by light microscopy, was not disturbed by either drug under the experimental conditions.
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7.
It is concluded that DC and OP do not interfere with active transport mechanisms but increase the permeability of the epithelium to sodium.
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Part of the results has been presented on occasion of the 14th and 15th Spring Meetings of the Deutsche Pharmakologische Gesellschaft at Mainz, Germany (Wanitschke et al., 1973; Wanitschke and Nell, 1974)
Supported by a grant from Sonderforschungsbereich 38, Membrane Research, Universität des Saarlandes
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Wanitschke, R., Nell, G., Rummel, W. et al. Transfer of sodium and water through isolated rat colonic mucosa under the influence of deoxycholate and oxyphenisatin. Naunyn-Schmiedeberg's Arch. Pharmacol. 297, 185–190 (1977). https://doi.org/10.1007/BF00499929
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DOI: https://doi.org/10.1007/BF00499929