Summary
Gamma-ray exposure was used as a mutagenic agent for mutant induction from a strain of Humicola lutea 72 producing acid proteases. The second stage of irradiation resulted in a double active mutant (H. lutea 120–8). Further treatment gave rise to a triple active mutant (H. lutea 120–5) with a maximal proteolytic activity of 1,800 μg tyrosine liberated from 2% casein/ml−1 culture filtrate/min−1 at pH 3,0 and 40°C. A change in the composition of proteolytic enzymes caused by gamma-ray exposure was established, using chromatography on DEAE-cellulose.
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Grigorov, I., Aleksieva, P., Djerova, A. et al. Selection of gamma-ray mutants from a strain of Humicola lutea 72, producing acid proteases. European J. Appl. Microbiol. Biotechnol. 17, 355–357 (1983). https://doi.org/10.1007/BF00499503
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DOI: https://doi.org/10.1007/BF00499503