Abstract
A simple and effective cryogenic procedure for the extraction of glycolytic intermediates from whole Drosophila has been developed. This procedure gives consistent results when a measure (µM/liter/OD260) is adopted which corrects for differences in extraction efficiency. Using this measure and a homozygous strain of D. mercatorum, there are no significant differences among extracts for the levels of any of the 15 glycolytic intermediate or energy molecules considered. The profile of means is consistent across experimental designs and instrument types. Coefficients of variation are well below 50% for most variables. The methodology presented has the statistical power to detect a mean change of 10 to 50% using an experimental design which requires as few as 32 observations. The estimated energy charge for resting Drosophila from these studies is the expected value of 0.86.
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This work was supported by NIH Grant 5-R01-AG-01804 and Department of Energy Contract E(11-1)2828.
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Clark, R.L., Brewer, G.J. & Sing, C.F. Studies of enzyme polymorphisms in the Kamuela population of Drosophila mercatorum. II. Evaluation of glycolytic intermediates. Biochem Genet 20, 1085–1103 (1982). https://doi.org/10.1007/BF00498934
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DOI: https://doi.org/10.1007/BF00498934