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Pathway of sodium moving from blood to intestinal lumen under the influence of oxyphenisatin and deoxycholate

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Summary

  1. 1.

    The transfer of 51CrEDTA and inulinsubstances which are distributed only in the extracellular space-across the rat colonic mucosa in vivo is increased by oxyphenisatin O (3.5 · 10−5 M) and deoxycholate D (3 · 10−3 M).

  2. 2.

    O and D do not change the size of the intra- and extracellular fluid compartments of the mucosa as measured with 51CrEDTA from the blood side. The sodium and potassium content of the mucosal tissue is not altered. Therefore the calculated intracellular concentrations of sodium and potassium remain constant.

  3. 3.

    The time course of the 22Na uptake into the mucosal epithelium is not influenced by O and D up to 5 min after i.v. injection. The specific activity of sodium, however, in the luminal fluid increases under the influence of O (twofold) and D (fivefold). The uptake of 22Na into the mucosal tissue after administration of 22Na into the intestinal lumen is not changed in presence of O and D.

  4. 4.

    We conclude that the net transport of sodium and water from blood to lumen under the influence of O and D occurs mainly via the intercellular way.

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, D-6650, Homburg (Saar), Federal Republic of Germany

    G. Nell, W. Forth, W. Rummel & R. Wanitschke

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  1. G. Nell
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  2. W. Forth
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  3. W. Rummel
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  4. R. Wanitschke
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Additional information

The results have already been presented in part on the occasion of the 13th spring meeting of the Deutsche Pharmakologische Gesellschaft, Mainz (Nell et al., 1972a), and on the II. Bile Acid Meeting, Freiburg i. Br., 1972 (Nell et al., 1972 b).

Supported by a grant from the Sonderforschungsbereich 38, Membrane Research, Universität des Saarlandes.

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Nell, G., Forth, W., Rummel, W. et al. Pathway of sodium moving from blood to intestinal lumen under the influence of oxyphenisatin and deoxycholate. Naunyn-Schmiedeberg's Arch. Pharmacol. 293, 31–37 (1976). https://doi.org/10.1007/BF00498868

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  • DOI: https://doi.org/10.1007/BF00498868

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