Summary
The dose dependence of the influence of deoxycholic acid (DOC) on fluid and sodium absorption, transmural potential difference (PD), permeability of 14C-erythritol and secretion of potassium and mucus (protein bound hexoses) was measured in the in vivo perfused rat colon. The following results were obtained:
-
1.
The threshold concentration for the inhibitory effect of DOC on fluid and sodium absorption is 2 mmol. In order to decrease PD and increase the colonic permeability for 14C-erythritol the same concentration was needed.
-
2.
In contrast, DOC stimulated potassium and mucus secretion even in a fourfold lower concentration (0.5 mmol). No difference in the responsiveness of the descending and ascending colon was observed.
-
3.
It is concluded that the identical dose dependency of the effect of DOC on fluid and sodium movement, PD, and permeability of the colonic mucosa is consistent with the interpretation that the secretagogue effect of DOC is mediated by an increase in permeability. However, because of their greater sensitivity, mucus and potassium secretion obviously are affected by a different mechanism. It is speculated that mucus and potassium are secreted together by the mucus producing cells of the colonic mucosa under the influence of DOC.
Similar content being viewed by others
References
Binder HJ, Dobbins JW, Racusen LC, Whiting DS (1978) Effect of propranolol on ricinoleic acid- and deoxycholic acid-induced changes of intestinal electrolyte movement and mucosal permeability. Gastroenterology 75:668–673
Camilleri M, Cooper BT, Chadwick VS (1978) Bile acid induced colonic secretion: increased mucosal cAMP or altered permeability? Gut 19: A 968
Camilleri M, Murphy R, Chadwick VS (1980) Dose-related effects of chenodeoxycholic acid in the rabbit colon. Dig Dis Sci 25:433–438
Chadwick VS, Gaginella TS, Carlson GL, Debongnie JC, Phillips SF, Hofmann AF (1979) Effect of molecular structure on bile acid induced alterations in absorptive function, permeability and morphology in the perfused rabbit colon. J Lab Clin Med 94:661–674
Crane CW (1965) Observations on the sodium and potassium of mucus from the large intestine. Gut 6:439–443
Farack UM, Nell G (1979) The influence of an adenylcyclase inhibitor on the choleratoxin-, deoxycholic acid- and bisacodyl-induced intestinal secretion in the rat. Naunyn-Schmiedeberg's Arch 308: Suppl R 27
Forstner G, Shih M, Lukie B (1973) Cyclic AMP and intestinal glycoprotein synthesis: The effect of β-adrenergic agents, theophyl-line, and dibutyryl cyclic AMP. Can J Physiol Pharmacol 51: 122–129
Forth W, Rummel W (1967) Resorptionshemmung, eine physiologische Wirkung von Gallensäuren? In: Hoffmann G (ed) Radioisotope in der Gastroenterologie. F. Schattauer, Stuttgart, pp 141–146
Forth W, Rummel W (1975) Activation and Inhibition of Intestinal Absorption by Drugs. In: Forth W, Rummel W (eds) International encyclopedia of pharmacology and therapeutics — Pharmacology of intestinal absorption: Gastrointestinal absorption of drugs, Section 39 B, vol 1. Pergamon Press, Oxford, pp 171–244
Forth W, Rummel W, Glasner H, Andres H (1966) Zur resorptionshemmenden Wirkung von Gallensäuren. Naunyn-Schmiedeberg's Arch Pharmakol Exp Pathol 254:364–380
Fry RJM, Staffeldt E (1964) Effect of a diet containing sodium deoxycholate on the intestinal mucosa of the mouse. Nature 203:1396–1398
Gaginella TS, Phillips SF, Dozois RR, Go VIW (1978) Stimulation of adenylate cyclase in homogenates of isolated intestinal epithelial cells from hamsters. Gastroenterology 74:11–15
Goerg KJ, Nell G, Specht W (1978) Correlation between the 51CrEDTA clearance and the secretion of fluid and electrolytes under the influence of deoxycholate (DC) in the rat colon. Naunyn-Schmiedeberg's Arch Pharmacol 302: Suppl R 1
Gordon SJ, Kinsey MD, Magen JS, Joseph RE, Kowlessar OD (1979) Structure of bile acids associated with secretion in the rat caecum. Gastroenterology 77:38–44
LaMont JT, Ventola A (1977) Stimulation of colonic glycoprotein synthesis by dibutyryl cyclic AMP and theophyllin. Gastroenterology 72:82–86
Lewin MR, El Masri SH, Clark CG (1979) Effects of bile acid on mucus secretion in the dog colon. Eur Surg Res 11:392–398
McJunkin B, Fromm H, Sarva RP, Amin P (1981) Factors in the mechanism of diarrhea in bile acid malabsorption: fecal pH-a key determinant. Gastroenterology 80:1454–1464
Mekhjian HS, Phillips SF (1970) Perfusion of the canine colon with unconjugated bile acids. Effect on water and electrolyte transport, morphology, and bile acid absorption. Gastroenterology 59: 120–129
Mekhjian HS, Phillips SF, Hofmann AF (1971) Colonic secretion of water and electrolytes induces by bile acids: perfusion studies in man. J Clin Invest 50:1569–1577
Murty VLN, Downs FJ, Pigman W (1978) Rat-colonic, mucus glycoprotein. Carbohydr Res 61:139–145
Nell G, Forth W, Rummel W, Wanitschke R (1976) Pathway of sodium moving from blood to the intestinal lumen under the influence of oxyphenisatin and deoxycholate. Naunyn-Schmiedeberg's Arch Pharmacol 293:31–37
Rummel W (1976) Wirkungen von Gallensäuren und Laxantien auf den mukosalen Transfer. Bull Schweiz Akad Med Wiss 32:233–250
Schoenfield LJ (1974) Medical therapy for gallstones. Gastroenterology 67:725–729
Sherr HP, Mertens RB (1979) Cholera toxin-induced glycoprotein secretion in rabbit small intestine. Gastroenterology 77:18–25
Simon B, Czygan P, Stiehl A, Kather H (1987) Human colonic adenylate cyclase: effects of bile acids. Eur J Clin Invest 8:321–323
Specht W (1977) Morphology of the intestinal wall. In: Kramer M, Lauterbach F (eds) Intestinal permeation. Excerpta Medica, Amsterdam Oxford, pp 4–40
Sullivan MF, Hulse EV, Mole RH (1965) The mucus-depleting action of bile in the small intestine of the irradiated rat. Br J Exp Pathol 46:235–244
Sund RB, Hillestad B (1981) Studies on hydragogue drugs: Effect of surfactants on cAMP levels in the rat jejunal mucosa in short time experiments in vivo. Acta Pharmacol Toxicol 49:110–115
Teem MV, Phillips SF (1972) Perfusion of the hamster jejunum with conjugated and unconjugated bile acids: inhibition of water absorption and effects on morphology. Gastroenterology 62:261–267
Yardley JH, Bayless TM, Luebbers EH, Halsted CH, Hendrix TR (1972) Goblet cell mucus in the small intestine. Findings after net fluid production due to cholera toxin and hypertonic solutions. J Hopkins Med J 131:1–10
Winzler RJ (1955) Determination of serum glycoproteins. Meth Biochem Anal 2:279–310
Author information
Authors and Affiliations
Additional information
This work was supported by the Sonderforschungsbereich 38 “Membrane Research”
Rights and permissions
About this article
Cite this article
Farack, U.M., Nell, G., Lueg, O. et al. Independence of the activation of mucus and potassium secretion on the inhibition of sodium and water absorption by deoxycholate in rat colon. Naunyn-Schmiedeberg's Arch. Pharmacol. 321, 336–340 (1982). https://doi.org/10.1007/BF00498523
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00498523