Histochemistry

, Volume 78, Issue 3, pp 391–397 | Cite as

The endocrine pancreas of a squamate reptile, the desert lizard (Chalcides ocellatus)

A histological and immunocytochemical investigation
  • M. El-Salhy
  • G. Abu-Sinna
  • E. Wilander
Article

Summary

The endocrine pancreas of the desert lizard (Chalcides ocellatus) was investigated histologically and immunocytochemically. The endocrine tissue was concentrated in the dorsal lobe, where it constituted about 7% of the total volume. In the ventral lobe the endocrine tissue formed approximately 1% of the total volume. Four endocrine cell types were observed in the pancreas of this species, namely insulin-, glucagon-, somatostain- and pancreatic polypeptide (PP)-immunoreactive cells. The volume occupied by these cells was 1, 1, 0.6 and 0.3% of the total volume of the pancreas, respectively. Insulin-immunoreactive cells were located in the islet centre and comprised 3% of dorsal and 0.2% of the ventral lobe volume. Glucagon cells occurred at the islet periphery and amounted to 3 and 0.2% of the volume of the dorsal and ventral lobes, respectively. Somatostatin-immunoreactive cells were located at the islet periphery as well as in between the exocrine parenchyma. They constituted 1 and 0.2% of the volume of the dorsal and ventral lobes, respectively. PP-immunoreactive cells occurred mainly among the exocrine parenchyma as solitary cells. They formed only 0.03% of the volume of the dorsal lobe. The corresponding figure in the ventral lobe was 0.6%.

Keywords

Glucagon Endocrine Cell Pancreatic Polypeptide Endocrine Pancreas Endocrine Tissue 

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References

  1. Bonner-Weir S, Weir GC (1979) The organization of the endocrine pancreas. A hypothetical unifying view of the phylogenetic differences. Gen Comp Endocrinol 38:28–37Google Scholar
  2. Buchan AMJ, Lance V, Polak JM (1982) The endocrine pancreas of Aligator mississippiensis. An immunocytochemical investigation. Cell Tissue Res 224:128Google Scholar
  3. El-Salhy M (1981) On the phylogeny of the gastro-enteropancratic (GEP) neuroendocrine system. Acta Univ Upsal 385:1–39Google Scholar
  4. El-Salhy M, Grimelius L (1981a) Histological and immunohistochemical studies of the endocrine pancreas of lizards. Histochemistry 72:237–247Google Scholar
  5. El-Salhy M, Grimelius L (1981 b) Immunohistochemical localization of gastrin C-terminus, gastric inhibitory peptide (GIP) and endorphin in the pancreas of lizards, with special reference to the hibernation periods. Regul Pept 2:97–111Google Scholar
  6. Erlandsen SL, Parsons JA, Burke JP, Orden DE van, Orden LS van (1975) A modification of the unlabelled antibody enzyme method using heterologous antisera for the light microscopic and ultrastructural localization of insulin, glucagon and growth hormone. J Histochem Cytochem 23:666–677Google Scholar
  7. Grimelius L (1968) A silver nitrate staining for 397-1 in human pancreatic islets. Acta Soc Med Upsal 73:243–270Google Scholar
  8. Rhoten WB, Smith PH (1978) Localization of four polypeptide hormones in the saurian pancreas. Am J Anat 151:595–602Google Scholar
  9. Scott HR (1952) Rapid staining of beta granules in pancreatic islets. Stain Technol 27:267–268Google Scholar
  10. Sternberger LS (1979) Immunocytochemistry. 2nd ed. John Wiley and Sons, New YorkGoogle Scholar
  11. Weibel ER (1963) Principles and methods for the morphometric study of the lung and other organs. Lab Invest 12:131–155Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • M. El-Salhy
    • 1
  • G. Abu-Sinna
    • 2
  • E. Wilander
    • 1
  1. 1.Department of PathologyUniversity HospitalUppsalaSweden
  2. 2.Department of Zoology, Faculty of ScienceAin-Shams UniversityCairoEgypt

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