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DNA-polymerase activity detected in situ

Relationship between incorporation of 3H-deoxytriphosphates and 3H-actinomycin D binding during the cell cycle in antheridial filaments of Chara vulgaris L. Effect of auxin and cytokinins

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Summary

DNA-polymerase activity during the cell cycle (S+G2+M+C type) in antheridial filaments cells of Chara vulgaris was studied using the autoradiographic method. Incorporation of 3H-deoxytriphosphates (3H-dTPs) during the whole of interphase indicates, that the cell cycle is not accompanied by distinct changes in enzyme activity. Incorporation of 3H-dTPs was also observed in spermatids and in early stages of spermatogenesis. Intensity of 3H-dTPs incorporation during interphase and spermatogenesis is similar to the intensity of 3H-actinomycin D (3H-AMD) binding. Auxin (IAA) and kinetin stimulate both 3H-AMD binding and 3H-dTPs incorporation; benzyladenine does not affect any of these processes. The in situ autoradiographic method of detecting DNA-polymerase activity reveals availability of DNA template for the enzyme rather than DNA polymerase activity itself.

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Authors and Affiliations

  1. Department of Plant cytology and Cytochemistry, Institute of Physiology and Cytology, University of Lodz, Banacha 12/16, PL-90-23, Lodz, Poland

    M. Godlewski

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Godlewski, M. DNA-polymerase activity detected in situ. Histochemistry 90, 309–313 (1988). https://doi.org/10.1007/BF00495975

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  • DOI: https://doi.org/10.1007/BF00495975

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