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Ultrastructural localization of acid protein polysaccharides and calcium in the vacuoles of the chicken thrombocyte

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Summary

The coexistence of acid protein polysaccharides and calcium in the vacuoles of chicken thrombocytes were studied by means of ultrahistochemical methods and electron probe X-ray microanalysis.

The thrombocytes possessed large vacuoles of a surface connected membrane system. After both ruthenium red staining and tannic acid fixation the innersurface coat of vacuoles was always strongly and continuously visualized. Electron microscopic X-ray microprobe analysis of antimonate precipitates in thrombocytes fixed in K-antimonate-OsO4 revealed calcium localization on the innersurface of vacuoles. From these facts it seems likely that the vacuoles of the surface connected membrane system may take part in the pool or the transport of calcium.

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Teikyo University, Kaga Itabashi-Ku, 173, Tokyo, Japan

    T. Daimon, K. Uchida & V. Mizuhira

  2. Department of Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

    T. Daimon, K. Uchida & V. Mizuhira

Authors
  1. T. Daimon
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  2. K. Uchida
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  3. V. Mizuhira
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Additional information

This investigation was supported in part by the grant from the Kazato Fundation

We are grateful to Professor T. Adesanya Ige Grillo of University of Ife, Nigeria for commenting on the manuscript

V. Mizuhira thanks for the Fundation of Mitsubishi

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Daimon, T., Uchida, K. & Mizuhira, V. Ultrastructural localization of acid protein polysaccharides and calcium in the vacuoles of the chicken thrombocyte. Histochemistry 52, 25–32 (1977). https://doi.org/10.1007/BF00495811

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  • DOI: https://doi.org/10.1007/BF00495811

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