Summary
The AFP-synthesizing cells were identified by ultrastructural localization of the antigen in regenerating liver of adult mice after CCl4 poisoning. An indirect immunoperoxidase method with rabbit anti-mouse AFP and peroxidase conjugates of anti-rabbit IgG or their Fab' was used. Good preservation of AFP and tissue structure, and sufficient permeability for the conjugates were obtained after 20′ prefixation of small liver specimens in 8% formaldehyde, −0.05% glutaraldehyde followed by 16 h fixation in 8% formaldehyde.
The intracellular localization of AFP observed in the light microscope in most cases corresponded to its synthesis and secretion. It was found in two cell types, both concentrated mainly in the perinecrotic zones and constituting only a small part of the whole cell population. Most of the AFP-producing cells were normal differentiated hepatocytes without any structural signs of damage. A few smaller cells with active AFP synthesis were present in some animals. By their ultrastructure they resembled the oval cells found during chemical hepatocarcinogenesis in rats.
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This work was partially supported by Immunological Unit of WHO
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Engelhardi, N.V., Baranov, V.N., Lazareva, M.N. et al. Ultrastructural localisation of alpha-fetoprotin (AFP) in regenerating mouse liver poisoned with CCL4 . Histochemistry 80, 401–407 (1984). https://doi.org/10.1007/BF00495425
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DOI: https://doi.org/10.1007/BF00495425