Skip to main content
SpringerLink
Log in

Structural Changes in the Liver in Post-Toxic Cirrhosis and Its Treatment with Oxidized Dextran. Immunohistochemical Research

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

In adult Wistar rats, post-toxic liver cirrhosis was induced by intraperitoneal injection of 50% oil solution of CCl4 and peroral administration of 6.5% aqueous solution of ethyl alcohol over 60 days. Histological examination of the liver revealed vacuolar degeneration and necrosis of hepatocytes, formation of false lobules, expression of collagens I and III periportally and in interlobular spaces, ascites, and hydrothorax. Then, oxidized dextran with a molecular weight of 40 kDa (2 ml of a 5% aqueous solution) was intraperitoneally injected every fourth day over 30 days. Against the background of treatment with oxidized dextran, the volume density of collagens I and III decreased by more than 2 times, the “collagen-producing” activity of fibroblasts decreased by 47%, and the number of fibroblasts decreased, including by the mechanism of apoptosis. The decrease in the collagen content in the liver of rats treated with oxidized dextran was apparently associated with blockade of collagen assembly due to the aldehyde—aldehyde interaction of tropocollagens and oxidized dextran.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tsuchida T, Friedman SL. Mechanisms of hepatic stellate cell activation. Nat. Rev. Gastroenterol. Hepatol. 2017;14(7):397-411. https://doi.org/10.1038/nrgastro.2017.38

    Article  CAS  PubMed  Google Scholar 

  2. Osipenko MF, Bikbulatova EA, Kholin SI. Liver cirrhosis. How to extend the life of patient? Med. Sovet. 2015;(13):76-79. Russian. https://doi.org/10.21518/2079-701X-2015-13-76-79

  3. Shkurupy VA, Kim LB, Kovner AV, Cherdantseva LA. Connective tissue and the problems of its pathological conditions. Byull. Sib. Med. 2017;16(4):75-85. Russian. https://doi.org/10.20538/1682-0363-2017-4-75-85

  4. Karpov MA, Klochin VD, Shkurupiy VA. Research on the oxidized dextran effect on fibrosis in the liver of rats with toxic hepatosis and liver cirrhosis. Sib. Nauch. Med. Zh. 2022;42(1):49-55. Russian. https://doi.org/10.18699/SSMJ20220105

  5. Shkurupy VA, Troitsky AV, Potapova OV, Luzgina NG. Patent EA No. 011718. The method of obtaining dialdehyde dextran. Byull. No. 02. Published April 28, 2009.

  6. Avtandilov GG. Fundamentals of Quantitative Pathology. Moscow, 2002. Russian.

  7. Shkurupy VA. Tuberculous Granulomatosis. Cytophysiology and Target Treatment. Moscow, 2007. Russian.

  8. Shkurupii VA, Kozyaev MA, Nadeev AP. Ultrastructural characteristics of type A epithelioid cells during BCG-granulomatosis and treatment with lysosomotropic isoniazid. Bull. Exp. Biol. Med. 2006;141(4):487-490. https://doi.org/10.1007/s10517-006-0206-0

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia

    M. A. Karpov, V. D. Klochin, A. P. Nadeev & V. A. Shkurupy

  2. Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia

    M. A. Karpov, A. P. Nadeev, V. A. Shkurupy & I. O. Marinkin

Authors
  1. M. A. Karpov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. D. Klochin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. P. Nadeev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. A. Shkurupy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. O. Marinkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. A. Karpov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 9, pp. 392-395, September, 2022

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Karpov, M.A., Klochin, V.D., Nadeev, A.P. et al. Structural Changes in the Liver in Post-Toxic Cirrhosis and Its Treatment with Oxidized Dextran. Immunohistochemical Research. Bull Exp Biol Med 174, 395–398 (2023). https://doi.org/10.1007/s10517-023-05715-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-023-05715-3

Keywords

Search

Navigation