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Immunolocalization of human cystatins in neutrophils and lymphocytes

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Summary

The endogenous inhibitors of cysteine proteinases, cystatin A and cystatin B, have been localized in sections of human liver by indirect immunofluorescence microscopy. Cystatin A was localized in neutrophils and cystatin B was found in lymphocytes. Both types of cell were present in the blood vessels and within the sinusoids between the parenchymal cells. Cystatin A was also found in some of the parenchymal cells surrounding the blood vesscts but, in contrast to the uniform fluorescence of the leucocytes, the staining in the parenchymal cells was granular and rather irregular.

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References

  • Brzin J, Kopitar M, Turk V (1983) Protein inhibitors of cysteine proteinases. I. Isolation and characterization of stefin, a cystolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes. Hoppe-Seyler's Z Phys Chem 364:1475–1480

    Google Scholar 

  • Bury AF (1981) Analysis of protein and peptide mixtures. Evaluation of three sodium dodecyl sulphate-polyacrylamide gel electrophoresis buffer systems. J Chromatogr 213:491–500

    Google Scholar 

  • Gauthier F, Pagano M, Esnard F, Mouray H, Engler R (1983) A heat stable low molecular weight inhibitor of lysosomal cysteine proteinases in human serum. Biochem Biophys Res Commun 110:449–455

    Google Scholar 

  • Green GDJ, Kembhavi AA, Davies ME, Barrett AJ (1984) Cystatin-like cysteine proteinase inhibitors from human liver. Biochem J 218:939–946

    Google Scholar 

  • Hawkes R, Niday E, Gordon J (1982) A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 119:142–147

    Google Scholar 

  • Järvinen M (1978) Purification and some characteristics of the human epidermal SH-protease inhibitor. J Invest Dermatol 71:114–118

    Google Scholar 

  • Järvinen M, Rinne A (1982) Human spleen cysteine proteinase inhibitor. Purification, fractionation into isoelectric variants and some properties of the variants. Biochim Biophys Acta 708:210–217

    Google Scholar 

  • Järvinen M, Räsänen O, Rinne A (1978) The low-molecular weight SH protease inhibitor in rat skin is epidermal. J Invest Dermatol 71:119–121

    Google Scholar 

  • Lenney JF, Liao JR, Sugg SL, Gopalakrishnan V, Wong HCH, Ouge KH, Chan PWH (1982) Low molecular weight inhibitors of cathepsins B, H and T in human serum, synovial fluid and CSF. Biochem Biophys Res Commun 108:1581–1587

    Google Scholar 

  • Nisonoff A, Wissler FC, Lipman LN, Woenley DL (1960) Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulphide bonds. Arch Biochem Biophys 89:230–244

    Google Scholar 

  • Ouchterlony O (1958) Diffusion-in-gel methods for immunological analysis. Prog in Allergy 5:1–78

    Google Scholar 

  • Porter RR (1959) The hydrolysis of rabbit γ-globulin and antibodies with crystalline papain. Biochem J 73:119–126

    Google Scholar 

  • Räsänen O, Järvinen M, Rinne A (1978) Localization of the human SH-protease inhibitor in the epidermis. Immunofluorescent studies. Acta Histochem 63:193–196

    Google Scholar 

  • Rinne A (1979) Epidermal SH-protease inhibitor. Occurrence in human and rat tissues and in human neoplasms. Acta Universitatis Ouluensis Ser D, Medica No. 41, p 18

  • Rinne A (1980) Epidermal SH-protease inhibitor in human neoplasma and their metastases. Pathol Res Pract 170:172–179

    Google Scholar 

  • Rinne A, Järvinen M, Räsänen O (1978) A protein reminiscent of the epidermal SH-protease inhibitor occurs in squamous epithelia of man and rat. Acta Histochem 63:183–192

    Google Scholar 

  • Rinne A, Järvinen M, Martikainen J, Alavaikko M, Räsänen O (1981) Über das Vorkommen des epidermalen SH-protease inhibitors im lymphatischen Gewebe. Verh anat Ges 75:573–574

    Google Scholar 

  • Rinne A, Alavaikko M, Järvinen M, Martikainen J, Karttunen T, Hopsu-Havu V (1983) Demonstration of immunoreactive acid cysteine-proteinase inhibitor in reticulum cells of lymph node germinal centres. Virch Arch (Cell Pathol) 43:121–126

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Turk V, Brzin J, Longer M, Ritonja A, Eropkin M, Borchart U, Machleidt W (1983) Protein inhibitors of cysteine proteinases. III. Amino-acid sequence of cysteine from chicken egg white. Hoppe-Seyler's Z Phys Chem 364:1487–1496

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Strangeways Laboratory, Worts Causeway, CB1 4RN, Cambridge, UK

    M. E. Davies & A. J. Barrett

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  1. M. E. Davies
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  2. A. J. Barrett
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Davies, M.E., Barrett, A.J. Immunolocalization of human cystatins in neutrophils and lymphocytes. Histochemistry 80, 373–377 (1984). https://doi.org/10.1007/BF00495420

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  • DOI: https://doi.org/10.1007/BF00495420

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