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Comparison of motor endings of the cat's muscle spindle stained for NADH-tetrazolium reductase and cholinesterase

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Summary

Cat muscle spindles were examined histochemically in serial transverse sections of tenuissimus muscles stained for ATPase, NADH-TR and ChE alternating sequentially. Motor nerve terminals on nuclear bag1, bag2 and nuclear chain intrafusal muscle fibers were identified in periodic sections stained for ChE. Intrafusal fiber regions that carried ChE-active areas were then examined in staining for NADH-TR. The motor endings on the three types of intrafusal fiber differed in their apparent histochemical content of both ChE and NADH-TR. The observations suggest that functional differences may exist among motor nerve terminals on the various intrafusal fiber types.

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References

  • Banks RW (1981) A histological study of the motor innervation of the cat's muscle spindle. J Anat (in press)

  • Banks RW, Barker D, Stacey MJ (1981) Structural aspects of fusimotor effects on spindle sensitivity. In: Taylor A, Prochazka A (eds) Muscle receptors and movement. Oxford University Press, New York, pp 5–16

    Google Scholar 

  • Banks RW, Harker DW, Stacey MJ (1977) A study of mammalian intrafusal muscle fibers using a combined histochemical and ultrastructural technique. J Anat 123:783–796

    Google Scholar 

  • Barker D (1974) The morphology of muscle receptors. In: Hunt CC (ed) Muscle receptors. Handbook of sensory physiology, vol III/2. Springer, Berlin Heidelberg New York, pp 1–190

    Google Scholar 

  • Barker, D, Bessou P, Jankowska, E, Pagès B, Stacey, MJ (1978) Identification of intrafusal muscle fibers activated by single fusimotor axons and injected with fluorescent dye in cat tenuissimus spindles. J Physiol 275:149–165

    Google Scholar 

  • Barker D, Stacey MJ, Adal MN (1970) Fusimotor innervation in the cat. Philos Trans R Soc Lond Ser B 258:315–346

    Google Scholar 

  • Barnard EA, Rogers AW (1967) Determination of the number, distribution and some in situ properties of cholinesterase molecules in the motor end plate, using labelled inhibitor methods. Ann N Y Acad Sci 144:584–612

    Google Scholar 

  • Boyd IA (1962) The structure and innervation of the nuclear bag muscle fiber system and the nuclear chain muscle fiber system in mammalian muscle spindles. Philos Trans R Soc Lond Ser B 245:81–136

    Google Scholar 

  • Boyd IA (1976a) The response of fast and slow nuclear bag fibers and nuclear chain fibers in isolated cat muscle spindles to fusimotor stimulation, and the effect of intrafusal contraction on the sensory endings. Q J Exp Physiol 61:203–254

    Google Scholar 

  • Boyd IA (1976b) The mechanical properties of dynamic nuclear bag fibers, static nuclear bag fibers and nuclear chain fibers in isolated cat spindles. Prog Brain Res 44:33–49

    Google Scholar 

  • Boyd IA, Gladden MH, McWilliam PN, Ward J (1977) Control of dynamic and static nuclear bag fibers and nuclear chain fibers by gamma and beta axons in isolated cat muscle spindles. J Physiol 265:133–162

    Google Scholar 

  • Brooke MH, Engel WK (1966) Nitro blue tetrazolium: Selective binding within striated muscle fibers. Neurology 16:799–806

    Google Scholar 

  • Buckley GA, Heaton J (1968) A quantitative study of cholinesterase in myoneural junctions from rat and guinea-pig extraocular muscles. J Physiol 199:743–749

    Google Scholar 

  • Buckley GA, Heaton J (1971) Cholinesterase activity of myoneural junctions from twitch and tonic muscles of the domestic fowl. Nature (New Biol) 231:154–155

    Google Scholar 

  • Coërs C, Durand J (1956) Données morphologiques nouvelles sur l'innervation des fuseaux neuromusculaires. Arch Biol (Liège) 67:685–715

    Google Scholar 

  • Emonet-Dénand F, Laporte Y (1974) Blôcage neuromusculaire sélectif des jonctions extrafusales des axones squeletto-fusimoteurs produit par leur stimulation répétitive à fréquence élevée. C R Acad Sci Ser D (Paris) 279:2083–2085

    Google Scholar 

  • Emonet-Dénand F, Laporte Y (1975) Proportion of muscle spindles supplied by skeletofusimotor axons (β-axons) in peroneus brevis muscle of the cat. J Neurophysiol 38:1390–1394

    Google Scholar 

  • Gladden MH (1981) The activity of intrafusal muscle fibers during central stimulation in the cat. In: Taylor A, Prochazka A (eds) Muscle receptors and movement. Oxford University Press, New York, pp 109–122

    Google Scholar 

  • Guth L, Samaha FJ (1970) Procedure for the histochemical demonstration of actomyosin ATPase. Exp Neurol 28:365–367

    Google Scholar 

  • Ip MC (1974) Some morphological features of the myoneural junctions in certain normal muscles of the rat. Anat Rec 180:605–616

    Google Scholar 

  • Kucera J (1980a) Motor nerve terminals of cat nuclear chain fibers studied by the cholinesterase technique. Neuroscience 5:403–411

    Google Scholar 

  • Kucera J (1980b) Motor innervation of the cat muscle spindle studied by the cholinesterase technique. Histochemistry 67:291–309

    Google Scholar 

  • Kucera J (1980c) Histochemical study of long nuclear chain fibers in the cat muscle spindle. Anat Rec 198:567–580

    Google Scholar 

  • Kucera J (1981a) Morphometric studies on tenuissimus muscle spindles in the cat. J Morphol 171 (in press)

  • Kucera J (1981b) Examination of chronically de-efferented cat muscle spindles for cholinesterase activity. Histochemistry 73:625–634

    Google Scholar 

  • Lannergren J, Smith RS (1966) Types of muscle fibers in toad skeletal muscle. Acta Physiol Scand 68:263–274

    Google Scholar 

  • Lehrer GM, Ornstein L (1959) A diazo coupling method for the electron microscopic localization of cholinesterase. J Biophys Biochem Cytol 6:399–406

    Google Scholar 

  • Leksell L (1945) The action potential and excitatory effects of the small ventral root fibers to skeletal muscles. Acta Physiol Scand 10 (Suppl 31):1–84

    Google Scholar 

  • Novikoff AB, Shin W, Drucker JC (1961) Mitochondrial localization of oxidative enzymes: staining results with two tetrazolium salts. J Biophys Biochem Cytol 9:47–61

    Google Scholar 

  • Ovalle WK, Smith RS (1972) Histochemical identification of three types of intrafusal muscle fibers in the cat and monkey based on the myosin ATPase reaction. Can J Physiol Pharmacol 50:195–202

    Google Scholar 

  • Padykula HA, Gauthier GF (1970) The ultrastructure of the neuromuscular junctions of mammalian red, white, and intermediate skeletal muscle fibers. J Cell Biol 46:27–41

    Google Scholar 

  • Peter JB, Barnard RS, Edgerton VR, Gilespie CA, Stempel KE (1972) Metabolic profiles of three fiber types of skeletal muscle in guinea-pigs and rabbits. Biochemistry 11:2623–2637

    Google Scholar 

  • Waerhaug O, Korneliussen H (1974) Morphological types of motor nerve terminals in rat hindlimb muscles, possibly innervating different muscle fiber types. Z Anat Entwickl Gesch 144:237–247

    Google Scholar 

  • Wong YC, Ip MC (1978) Structural differences of pale and strongly stained motor end-plates of the rat diaphragm. Am J Anat 152:529–538

    Google Scholar 

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Kucera, J. Comparison of motor endings of the cat's muscle spindle stained for NADH-tetrazolium reductase and cholinesterase. Histochemistry 74, 95–106 (1982). https://doi.org/10.1007/BF00495055

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