Histochemistry

, Volume 86, Issue 2, pp 147–157 | Cite as

Monoclonal antibodies demonstrating GABA-like immunoreactivity

  • C. Matute
  • P. Streit
Article

Summary

Mouse monoclonal antibodies (mAb) to GABA were developed following immunization with GABA coupled to bovine serum albumin (GABA-BSA). The selection of hybridoma cell lines producing antibodies which reacted with GABA-BSA but not with glutamate-BSA conjugates as well as the characterization of chosen mAb was performed by enzyme linked immunosorbent assays (ELISA). The five mAb selected were all of the IgG class and displayed different patterns of crossreactivities with the amino acid- and dipeptide-BAS conjugates tested. MAb 3A12 reacted approximately 4,000 times better with GABA-BSA than with β-alanine-BSA conjugates according to serial dilution experiments of the antibody in ELISA. Immunoreactivity was even lower for other conjugates tested including glycine-, taurine-, glutamate-, and glutamine-BSA. Immunohistochemical results in rat and chicken brain indicated that the patterns of GABA-like immunoreactivity observed with these mAb were consistent with the available information on the distribution of GABA-containing neurons.

Keywords

Albumin Monoclonal Antibody Glutamate Bovine Serum Albumin Glycine 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • C. Matute
    • 1
  • P. Streit
    • 1
  1. 1.Brain Research InstituteUniversity of ZürichZürichSwitzerland

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