Summary
Postnatal development of autonomic nerves of major cerebral arteries was histochemically studied in mice from one day to five months of age. For demonstration of aminergic nerves the glyoxylic acid method was used, while for cholinergic nerves Karnovsky and Roots' technique was utilized consecutively on the same whole mount preparations. The results obtained were as follows:
-
1)
In one-day-old mice a few aminergic nerves were seen while cholinergic nerves were scarcely observed. The cholinergic nerves were clearly observed in one-week-old mice. Then, both nerves increased rapidly in the first 2 weeks with a slight delay of maturation in the latter. They completed development between 3 and 4 weeks.
-
2)
Longitudinal and circular distributional patterns were observed for the both nerves; the former pattern developed earlier than the latter.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Axelsson S, Björklund A, Falck B, Lindvall O, Svensson LA (1973) Glyoxylic acid condensation: a new fluorescence method for the histochemical demonstration of biogenic monoamines. Acta Physiol Scand 87:57–62
Butcher LL, Hodge GK (1976) Postnatal development of acetylcholinesterase in the caudate-putamen nucleus and substantia nigra of rats. Brain Res 106:223–240
Champlain J De, Malmfors T, Olson L, Sachs C (1970) Ontogenesis of peripheral adrenergic neurons in the rat: pre- and postnatal observations. Acta Physiol Scand 80:276–288
Davis DC, Navaratham V (1979) Development of adrenoceptive and cholinoceptive responsiveness in the rat iris. Exp Eye Res 29:203–210
Edvinsson L, Lindvall M, Nielsen KC (1973) Are brain vessels innervated also by central (nonsympathetic) adrenergic neurones? Brain Res 63:496–499
Eränkö L (1972) Postnatal development of histochemically demonstrable catecholamines in the superior cervical ganglion of the rat. Histochem J 4:225–236
Eränkö L (1972) Ultrastructure of the developing sympathetic nerve cell and the storage of catecholamines. Brain Res 46:159–175
Falck B, Mchedlichvili GL, Owman CH (1965) Histochemical demonstration of adrenergic nerves in cortex-pia of rabbit. Acta Pharmacol Toxicol 23:133–142
Hamori J, Dyachkova LN (1964) Electron microscope studies on developmental differentiation of ciliary ganglion synapses in the chick. Acta Biol Acad Sci Hung 15:213–230
Hervonen A (1971) Development of catecholamine-storing cells in human fetal paraganglia and adrenal medulla. Acta Physiol Scand 83 Suppl 368:1–94
Imai H, Nakai K, Kamei I, Itakura T, Komai N, Nagai T, Kimura H, Imamoto K, Maeda T (1980) Simultaneous detection method of amine and acethlcholinesterase containing nerve fibres in pial vessels- 1. Light microscopic study. Cell Mol Biol 26:201–206
Iwayama T (1970) Ultrastructural changes in the nerves innervating the cerebral artery after sympathectomy. Z Zellforsch 109:465–480
Iwayama T, Furness JB, Burnstock G (1970) Dual adrenergic and cholinergic innervation of the cerebral arteries of the rat. An ultrastructural study. Circ Res 26:635–646
Joó F, Várkonyi T, Csillik B (1967) Developmental alterations in the histochemical structures o brain capillaries: A Histochemical contribution to the problem of the blood-brain barrier. Histochemie 9:140–148
Karnovsky MJ, Roots L (1964) A ‘direct-coloring’ thiocholine method for cholinesterases. J Histochem Cytochem 12:219–221
Kobayashi S, Tsukahara S, Sugita K, Nagata T (1981) Adrenergic and cholinergic innervation of rat cerebral arteries, Consecutive demonstration on whole mount preparations. Histochemistry 70:129–138
Lind NA, Shinebourne E (1970) Studies on the development of the autonomic innervation of the human iris. Br J Pharmacol 38:462P
Machado ABM (1971) Electron microscopy of developing sympathetic fibres in the rat pineal body. The formation of granular vesicles. In: Eränkö O (ed) Histochemistry of nervous transmission. Elsevier, Amsterdam (Progress in Brain Research, Vol 34, pp 171–185)
Nelson E, Rennels M (1970) Neuromuscular contacts in intracranial arteries of the cat. Science 167:301–302
Nielsen KG, Owman CH, Sporrong B (1971) Ultrastructure of the autonomic innervation apparatus in the main pial arteries of rats and cats. Brain Res 27:25–32
Nielsen KG, Owman CH (1967) Adrenergic innervation of pial arteries related to the circle of Willis in the cat. Brain Res 6:773–776
Panula P, Rechardt L (1979) The development of histochemically demonstrable cholinesterases in the rat neostriatum in vivo and in vitro. Histochemistry 64:35–50
Pick J, Gerdin C, Delemos C (1964) An electron microscopical study of developing sympathetic neurons in man. Z Zellforsch 62:402–415
Sato S (1966) An electron microscopic study on the innervation of the intracranial artery of the rat. Am J Anat 118:873–890
Tervo T (1977) Consecutive demonstration of nerves containing catecholamine and acetylcholinesterase in the rat cornea. Histochemistry 50:291–299
Wechsler W, Schmekel L (1967) Elektronenmikroskopische Untersuchung der Entwicklung der vegetativen (Grenzstrang-) und spinalen Ganglien bei Gallus domesticus. Acta Neuroveg (Wien). 30:427–444
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kobayashi, S., Tsukahara, S., Sugita, K. et al. Histochemical studies on the postnatal development of autonomic nerves in mice cerebral arteries. Histochemistry 73, 15–20 (1981). https://doi.org/10.1007/BF00493128
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00493128