Summary
Uptake and localization of mercury was studied in rats orally intoxicated with inorganic mercury.
By atomic absorption spectrophotometry large quantitative differences were found between test and control animals, particularly relating to blood, kidney and brain.
By histochemical demonstration of heavy metals the uptake in the CNS was shown to occur particularly within the cytoplasm of large neurons in the cortex, pons and basal ganglia but also in other neurons, to some extent in the choroid plexus and the vessel walls, and least in the white matter. No lesions were detectable by light microscopy. The mercury was mostly in the methylated form, something that may be explained by gastrointestinal methylation by bacteria. A similar mechanism can be expected in human chronic inorganic mercury poisoning.
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Abdulla, M., Arnesjö, B., Ihse, I.: Methylation of inorganic mercury in experimental jejunal blind-loop. Scand. J. Gastroent. 8, 565–567 (1973)
Abdulla, M.: Experimental feeding of inorganic mercury to rainbow trouts. Unpublished (1973)
Brun, A., Brunk, U.: Histochemical evidence for lysosomal uptake of lead in tissue cultured fibroblasts. Histochemie 29, 140–146 (1972)
Brun, A., Brunk, U.: Heavy metal localization and age related accumulation in the rat nervous system. A histochemical and atomic absorption spectrophotometric study. Histochemie 34, 333–342 (1973)
Brunk, U., Brun, A., Sköld, G.: Histochemical demonstration of heavy metals with the sulfide silver method. A methodological study. Acta histochem. (Jena) 3, 345–357 (1968)
Cavanagh, J. B., Chen, F. C. K.: The effects of methylmercury-dicyandiamide on the peripheral nerves and spinal cord of rats. Acta Neuropath. 19, 208–215 (1971)
Chang, L. W., Dudley, A. W. Jr., Ware, R. A.: Blood barrier dysfunction following mercury intoxication. J. Neuropath. Exp. Neurol. 1, 175 (1974)
Chang, L. W., Dudley, A. W. Jr., Ware, R. A.: Mitochondrial changes in neurons following mercury intoxication. J. Neuropath. exp. Neurol. 1, 175–176 (1974)
Chang, C. W., Hartmann, H. A.: Ultrastructural studies of the nervous system after mercury intoxication. I. Pathological changes in the nerve cell bodies. Acta Neuropath. 20, 122–138 (1972)
Fehling, C., Abdulla, M., Brun, A., Dictor, M., Schütz, A., Skerfing, S.: Methyl mercury poisoning in the rat: A combined neurological, chemical and histopathological study. Toxicol. Appl. Pharmacol. 33, 27–37 (1975)
Friberg, L., Vostal, I.: Mercury in the environment CRC-PRESS Cleveland Ohio (1972)
Grant, C. A.: Pathology of experimental methylmercury intoxication: Some problems of exposure and response. Chap. 17. Mercury, Mercurials and Mercaptans. 294–312 (1972)
Jensen, S., Jernelöv, A.: Biological methylation of mercury in aquatic organisms. Nature (London) 233, 753–754 (1969)
Schütz, A.: A method for determination of submicrogram quantities of mercury in blood, urine and other biological material. Report 691020 from Department of Occupational Medicine University Hospital, S-221 85 Lund (1969)
Vonk, J. W., Sijpesteün, A. K.: Studies on the methylation of mercuric chloride by pure cultures of bacteria and fungi. Antonie v. Leeuwenhoek 39, 505–513 (1973)
Westöö, G.: Determination of methylmercury salts in various kinds of biological material. Acta chem. scand. 22, 2277–2280 (1968)
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Brun, A., Abdulla, M., Ihse, I. et al. Uptake and localization of mercury in the brain of rats after prolonged oral feeding with mercuric chloride. Histochemistry 47, 23–29 (1976). https://doi.org/10.1007/BF00492990
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DOI: https://doi.org/10.1007/BF00492990