Summary
Neodymium nitrate has been used instead of lead nitrate in the modified Wachstein-Meisel incubation solution. Neodymium reacts easily with the anorganic phosphate groups. The rare earth metal has strong electron scattering property. It was used in 3,0 mM concentration in the incubation solution. The histochemical precipitate obtained, appeared to be selective and was well localizable. Neodymium nitrate seems to be suitable for the electron microscopic histochemical demonstration of hydrolytic enzymes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Geyer, G.: Ultrahistochemie, Jena: VEB Gustav Fischer Verlag 1973
Grant, W. M., Kern, H. L.: Rare earths in biochemical and medical research. ORINS 12, 346–351 (1956)
Goodenough, D. A., Revel, J. P.: A fine structural analysis of intercellular junction in the mouse liver. J. Cell. Biol. 45, 272–290 (1970)
Haley, J. H.: Pharmacology and toxicology of the rare earth elements. J. Pharm. Sci. 54, 663–670 (1965)
Harper, P. V., Kyker, G. C., Anderson, E. B.: Rare earths in biochemical and medical research. Edit by: Orins 1956
Hori, S. H.: On the validity of the Wachstein-Meisel method for adenosine triphosphatase. Acta Histochem. Cytochem. 1, 136–138 (1968)
Kerpel-Fronius, S., Hajos, F.: The use of ferricyanid for the light and electron microscopic demonstration of succinic dehidrogenase activity. Histochemie 14, 343–351 (1968)
Kluss, B. C.: Electron microscopy of the macronucleus of Euplotes eurystomus. J. Cell. Biol. 13, 462–465 (1962)
Mizutani, A., Barnett, R. J.: Fine structural demonstration of phosphatase activity at pH 9. Nature (Lond.) 206, 1001 (1965)
Moeller, T.: The chemistry of lanthanides. New York: Rheinhold Publishing Corp. 1963
Mogilevskaya, O. Y., Raikhlin, N. T.: Toxicology of rare metals. Gosudar. Med. Lit. 41, 195–208 (1963)
Moses, H. L., Rosenthal, A. S.: On significance of lead catalyzed hydrolysis of nucleoside phosphatases in histochemical systems. J. Histochem. Cytochem. 15, 354–355 (1967)
Moses, H. L., Rosenthal, A. C.: Pitfalls in the use lead ion for histochemical localization of nucleoside phosphatases. J. Histochem. Cytochem. 16, 530–539 (1969)
Novikoff, A. B.: Enzyme localization with Gomori type lead phosphat procedures: real of artifact. J. Histochem. Cytochem. 18, 366–367 (1970)
Rayns, D. G., Simpson, F. O., Ledingham, J. M.: Ultrastructure of desmosomes in mammalian intercalated disc; appearances after lanthanium treatment. J. Cell. Biol. 42, 316–322 (1969)
Revel, J. P., Karnovsky, M. J.: Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell. Biol. 33, C7 (1967)
Schulze, W., Wollenberger, A.: Elektronmikroskopischer Nachweis von mitochondrialer Adenosintriphosphatase-Aktivität in tierischen Zellen. Histochemie. 5, 417–429 (1965)
Schulze, W., Wollenberger, A.: Zytochemische Localization und Charakterisierung von phosphatspaltenden Fermenten in sarcotubulären System quergestreiften Muskeln. Histochemie. 10, 140–153 (1967)
Schulze, W., Wollenberger, A.: Zytochemische Localisation und Differenzierung von Na+−K+ und anderer membranständiger ATP-ase aktivität im Herzmuskel. Histochemie. 19, 302–318 (1968)
Somogyi, E., Sotonyi, P., Bujdoso, Gy.: Electronmicroscopic histochemical examination of potassium-sodium dependent myocardial ATP-ase activity. Acta histochem. (Jena) 40, 64–72 (1971)
Somogyi, E., Sotonyi, P., Bujdoso, Gy.: Enzymatic-histochemical examination of magnesium-dependent myocardial ATP-ase activity under the electronmicroscope. Acta histochem. (Jena) 39, 286–297 (1971)
Somogyi, E., Sotonyi, P., Bujdoso, Gy.: Electronmicroscopic histochemical demonstration of sarcotubular ATP-ase in the myocardium. Acta histochem. (Jena) 43, 302–308 (1972)
Spedding, F. M., Daane, A. M.: The rare earths. New York and London: John Wiley and Sons. Inc. 1961
Stoeckenius, W.: Electronmicroscopy of DNA-molecules stained with heavy metals. J. biophys. biochem. Cytol. 11, 297–310 (1961)
Szinay, Gy., Sotonyi, P.: Morphological changes due to anticoagulants of the rare earth metal type, 7th Congress of International Academy of Legal Medicine and of Social Medicine, Abstract. 297 (1967)
Szinay, Gy., Sotonyi, P.: Morphological date to anticoagulants of rare earth metal type. Congr. hung. Med. Abstr. 31 (1968)
Szinay, Gy., Varga, T.: Experimental glomeluropathy due to rare earth metal complexes. Acta morph. Acad. Sci. hung. Suppl. XIV, 110 (1973)
Tice, L. W.: Studies on lead inihibition of adenosine triphosphatasese. III. Internat. Congr. Histochem. Cytochem. New York. 270–271 (1968)
Tice, L. W.: Lead adenosine triphosphate complexes in the ATP-ase activity. J. Histochem. Cytochem. 17, 85–94 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sótonyi, P., Somogyi, E. & Kerényi, N.A. Use of neodymium nitrate in electron microscopic histochemical demonstration of ATP-ase. Histochemistry 42, 265–269 (1974). https://doi.org/10.1007/BF00492659
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00492659