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Histochemistry

, Volume 87, Issue 4, pp 293–300 | Cite as

Immunohistochemical detection of human epidermal growth factor in submandibular glands and their tumors using a polyclonal antiserum and a monoclonal antibody

  • K. Tsukitani
  • Y. Tatemoto
  • Y. Noda
  • M. Mori
  • T. Hayashi
  • K. Kato
Article

Summary

We applied immunohistochemical procedures to detect hEGF in salivary glands and pleomorphic adenomas of salivary-gland origin using a polyclonal hEGF antiserum and a monoclonal antibody against hEGF synthesized by applying the synthetic gene technique using Escherichia coli. In normal salivary glands, hEGF was mainly localized in the ductal system (i.e., intercalated, striated, and excretory ducts). The staining intensity and intracellular localization exhibited some variation depending on the fixative used. When a polyclonal hEGF antiserum was used for immunostaining, slight background staining was observed in sections prepared using the fixatives tested. Therefore, precise localization of hEGF was obtainable only in formalin-fixed sections using the monoclonal antibody against hEGF. In pleomorphic adenomas, positive hEGF staining was seen on the luminal side of tumors and in cells of ductal origin; no reactivity was present on the outer side of tumors or in cells of myoepithelial origin. Occasionally, long, spindleshaped tumor cells and chondroidally changed tumor cells also exhibited positive staining for hEGF.

Keywords

Salivary Gland Submandibular Gland Pleomorphic Adenoma Outer Side Ductal System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • K. Tsukitani
    • 1
  • Y. Tatemoto
    • 1
  • Y. Noda
    • 1
  • M. Mori
    • 1
  • T. Hayashi
    • 2
  • K. Kato
    • 2
  1. 1.Department of Oral SurgeryAsahi University School of DentistryGifuJapan
  2. 2.Central Research LaboratoriesWakunaga PharmaceuticalsHiroshimaJapan

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