Skip to main content
SpringerLink
Log in

Demonstration of GABA-like immunoreactivity in myenteric plexus of frog stomach

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The GABAergic innervation of the frog stomach was studied by means of an indirect immunohistochemical method. Whole mount preparations were obtained from frog stomachs after the animals had been perfused with a mixture of picric acid, glutaraldehyde and glacial acetic acid. Samples were incubated with an antiserum specific for GABA coupled to BSA with glutaraldehyde. Anti-rabbit IgG-HRP was processed by the two step method (Eckert and Ude 1983).

GABA-positive varicose fibers and also nerve cell bodies were revealed within the myenteric plexus. The density of GABA-immunoreactive neurons was not higher than 4–8 cell/cm2, which is approximately 1% of the total nerve cell number in the myenteric plexus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baetge G, Gershon D (1986) GABA in the PNS: Demonstration in enteric neurons. Brain Res Bull 16:421–424

    Google Scholar 

  • Barthó L, Holzer P (1985) Search for a physiological role of substance P in gastrointestinal motility. Neuroscience 16:1–32

    Google Scholar 

  • Blechschmidt K, Eckert M, Penzlin H (1988) Simultaneous localization of a classical neurotransmitter (GABA) and a peptide transmitter candidate (proctolin) in the nervous system of the cockroach, Periplaneta americana. Acta Histochem 35S:127–135

    Google Scholar 

  • Cherubini E, North RA (1984) Inhibition of calcium spikes and transmitter release by y-aminobutiric acid in the guinea-pig myenteric plexus. Br J Pharmacol 82:101–105

    Google Scholar 

  • Davanger S, Ottersen OP, Storm-Mathisen J (1987) Immunocytochemical localization of GABA in cat myenteric plexus. Neurosci Lett 73:27–32

    Google Scholar 

  • Eckert M, Ude J (1983) Immunocytochemical techniques for the identification of peptidergic neurons. In: Strausfeld NJ (ed) Functional neuroanatomy. (Springer Series in Experimental Entomology) Springer, Berlin Heidelberg New York Tokyo, pp 267–301

    Google Scholar 

  • Gábriel R, Halasy K, Csoknya M (1987) Cytochemical detection of NADH-diaphorase positive nerve cells in the alimentary canal of frog. Acta Biol (Szeged) 33:85–95

    Google Scholar 

  • Halasy K, Eckert M, Fekete É, Gábriel R, Benedeczky I (1988) Comparative cytochemical studies on the enteric nervous system. Eur J Neurosci 258

  • Jensen J, Holmgren S, Jönsson A-C (1987) Substance P-like immunoreactivity and effects of tachykinins in the intestine of the Atlantic cod, Gadus morhua. J Auton Nerv Syst 20:25–33

    Google Scholar 

  • Jessen KR, Mirsky R, Dennison ME, Burnstock G (1979) GABA may be a neurotransmitter in the vertebrate peripheral nervous system. Nature 281:71–74

    Google Scholar 

  • Jessen KR, Hills JM, Saffrey MJ (1986) Immunohistochemical demonstration of GABAergic neurons in the enteric nervous system. J Neurosci 6:1628–1634

    Google Scholar 

  • Krantis A, Kerr DIB (1981) Autoradiographic localization of 3H-gamma-aminobutyric acid in the myenteric plexus of guinea-pig small intestine. Neurosci Lett 23:263–268

    Google Scholar 

  • Maggi CA, Manzini S, Meli A (1984) Evidence that GABAa receptors mediate relaxation of rat duodenum by activating intramural nonadrenergic noncholinergic neurones. J Auton Pharmacol 4:77–85

    Google Scholar 

  • Ong J, Kerr DIB (1984) Potentiation of GABAa receptor-mediated responses by barbiturates in the guinea pig ileum. Eur J Pharmacol 103:327–332

    Google Scholar 

  • Rashid S (1972) The nature of the parasympathetic nerve fibers of the stomach of Rana temporaria. Arch Int Pharmacodynther 195:247–251

    Google Scholar 

  • Saffrey MJ, Marcus N, Jessen KR, Burnstock G (1983) Distribution of neurons with high-affinity uptake sites for GABA in the myeteric plexus of the guinea pig, rat and chicken. Cell Tissue Res 234:231–235

    Google Scholar 

  • Schcuermann DW, Stach W, De Groodt-Lassel MHA, Timmermans J-P (1987) Calcitonin gene-related peptide in morphologically well-defined type II. neurons of the enteric nervous system in the porcine small intestine. Acta Anat 129:325–328

    Google Scholar 

  • Sternini C (1988) Structural and chemical organization of the myenteric plexus. Annu Rev Physiol 50:81–93

    Google Scholar 

  • Tanaka C, Taniyama K (1985) Substance P provoked y-aminobutyric acid release from the myenteric plexus of the guinea pig small intestine. J Physiol (London) 362:319–329

    Google Scholar 

  • Taniyama K, Miki Y, Tanaka C (1982) Presence of y-aminobutyric acid and glutamic acid decarboxylase in Auerbach's plexus of cat colon. Neurosci Lett 29:53–56

    Google Scholar 

  • Taniyama K, Kusunoki M, Saito N, Tanaka C (1983) GABA evoked ACh release from isolated guinea pig ileum. Life Sci 32:2349–2353

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, Attila József University, Egyetem u. 2, P.O. Box 659, H-6722, Szeged, Hungary

    R. Gábriel

  2. Department of Animal Physiology, Friedrich Schiller University, Jena, Germany

    M. Eckert

Authors
  1. R. Gábriel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Eckert
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gábriel, R., Eckert, M. Demonstration of GABA-like immunoreactivity in myenteric plexus of frog stomach. Histochemistry 91, 523–525 (1989). https://doi.org/10.1007/BF00492526

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00492526

Keywords

Search

Navigation