Skip to main content
Log in

Pancreatic APUD cells in older chick embryos, with special reference to their identity

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Pancreatic APUD cells showing formaldehyde-induced fluorescence in Black Australorp chick embryos of nine to eighteen days of incubation, proved, on subsequent staining and silver impregnation, to be A, B, D and, from sixteen days, enterochromaffin (EC) cells. EC and D cells were scattered in the exocrine parenchyma, the latter cells increasing with time. Some groups of B cells were associated with large A islets from the ninth day of incubation onwards. The composition of A islets (A and some D cells) and B islets (B and some D cells) and the distribution attained (A islets in the splenic and third lobes; B islets in all lobes), accords with the situation reported for adults.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Andrew, A.: APUD cells in the endocrine pancreas and the intestine of chick embryos. Gen. comp. Endocr. 26, 485–495 (1975)

    Google Scholar 

  • Andrew, A.: An experimental investigation into the possible neural crest origin of pancreatic APUD (islet) cells. J. Embryol. exp. Morph. 35, 577–593 (1976)

    Google Scholar 

  • Cegrell, L.: The occurrence of biogenic amines in the mammalian endocrine pancreas. Acta physiol. scand., Suppl. 314, 40–41 (1968a)

    Google Scholar 

  • Cegrell, L.: Uptake of L-Dopa in the pancreatic islets of albino mice. Acta physiol. scand., Suppl. 314, 35–39 (1968b)

    Google Scholar 

  • Cegrell, L.: The occurrence of biogenic amines in the mammalian endocrine pancreas. Acta physiol. scand., Suppl. 314, 6–7 (1968c)

    Google Scholar 

  • Cegrell, L., Falck, B., Rosengren, E.: Monoamines in the pig pancreas with special reference to the endocrine part. Acta physiol scand., Suppl. 314, 8–14 (1968)

    Google Scholar 

  • Dieterlen-Lièvre, F.: Demonstration de l'activite precoce des cellules A du pancréas chez l'embryon de poulet. C.R. Acad. Sci. (Paris) 256, 1597–1599 (1963)

    Google Scholar 

  • Dieterlen-Lièvre, F.: Etude morphologique et expérimentale de la différenciation du pancréas chez l'embryon de poulet. Bull. Biol. France Belg. 99, 3–116 (1965)

    Google Scholar 

  • Dieterlen-Lièvre, F., Beaupain, D.: Etude immunocytologique de la différenciation du pancréas endocrine chez l'embryon de poulet. I. Îlots à insuline. Gen. comp. Endocr. 22, 62–69 (1974)

    Google Scholar 

  • Enemar, A., Falck, B., Håkanson, R.: Observations on the appearance of norepinephrine in the sympathetic nervous system of the chick embryo. Develop. Biol. 11, 268–283 (1965)

    Google Scholar 

  • Epple, A., Lewis, T.L.: Comparative histophysiology of the pancreatic islets. Amer. Zool. 13, 567–590 (1973)

    Google Scholar 

  • Falck, B.: Observations on the possibilities of the cellular localization of monoamines by the fluorescence method. Acta physiol. scand. 56, Suppl. 197, 1–25 (1962)

    Google Scholar 

  • Falck, B., Hellman, B.: Evidence for the presence of biogenic amines in pancreatic islets. Experientia (Basel) 19, 139–140 (1963)

    Google Scholar 

  • Falck, B., Owman, C.: 5-Hydroxytryptamine and related amines in endocrine cell systems. Advanc. Pharmacol. 6, 211–231 (1968)

    Google Scholar 

  • Hellerström, C., Hellman, B.: Some aspects of silver impregnation of the islets of Langerhans in the rat. Acta endocr. (Kbh.) 35, 518–532 (1960)

    Google Scholar 

  • Hellman, B., Hellerström, C.: The islets of Langerhans in ducks and chickens with special reference to the argyrophil reaction. Z. Zellforsch. 52, 278–290 (1960)

    Google Scholar 

  • Jennings, B.M.: Aldehyde-fuchsin staining applied to frozen sections for demonstrating pituitary and pancreatic beta cells. J. Histochem. Cytochem. 13, 328–333 (1965)

    Google Scholar 

  • Legg, P.G.: Fluorescence studies on structures and endocrine cells in the pancreas of the cat. Z. Zellforsch. 88, 487–495 (1968)

    Google Scholar 

  • Levene, C., Feng, P.: Critical staining of pancreatic alpha granules with phosphotungstic acid haematoxylin. Stain Technol. 39, 39–41 (1964)

    Google Scholar 

  • Mikami, S.I., Mutoh, K.: Light and electron microscopic studies of the pancreatic islet cells in the chicken under normal and experimental conditions. Z. Zellforsch. 116, 205–227 (1971)

    Google Scholar 

  • Mikami, S.I., Ono, K.: Glucagon deficiency induced by extirpation of alpha islets of the fowl pancreas. Endocrinol. 71, 464–473 (1962)

    Google Scholar 

  • Owman, C., Håkanson, R., Sundler, F.: Occurrence and function of amines in endocrine cells producing polypeptide hormones. Fed. Proc. 32, 1785–1791 (1973)

    Google Scholar 

  • Pearse, A.G.E.: Histochemistry. Theoretical and applied, p. 919. London: J.&A. Churchill 1960

    Google Scholar 

  • Pearse, A.G.E.: Common cytochemical properties of cells producing polypeptide hormones with particular reference to calcitonin and the thyroid C cells. Vet. Rec. 79, 587–590 (1966)

    Google Scholar 

  • Pearse, A.G.E., Polak, J.M., Heath, C.M.: Development, differentiation and derivation of the endocrine polypeptide cells of the mouse pancreas. Immunofluorescence, cytochemical and ultrastructural studies. Diabetologia 9, 120–129 (1973)

    Google Scholar 

  • Pentila, A., Mustakallio, A.: Monoamine oxidase activity as related to the 5-hydroxytryptamine stores in the chicken duodenum during development. Acta histochem. (Jena) 31, 1–13 (1968)

    Google Scholar 

  • Quickel, K.E., Feldman, J.M., Lebovitz, H.E.: Inhibition of insulin secretion by serotonin and dopamine: species variation. Endocrinology 89, 1295–1302 (1971)

    Google Scholar 

  • Romanoff, A.L.: The avian embryo, p. 527. New York: Macmillan 1960

    Google Scholar 

  • Sandstrom, R.H.: The differentiation of hepatic and pancreatic tissues of the chick in chorio-allantoic grafts. Physiol. Zool. 7, 226–246 (1934)

    Google Scholar 

  • Slanina, P., Tjälve, H.: Uptake of 3H-atropine in pancreatic islets and the parafollicular cells of the thyroid in mice shown by micro-autoradiography. Acta physiol. scand. 84, 254–260 (1971)

    Google Scholar 

  • Smith, P.H.: Pancreatic islets of the coturnix quail. A light and electron microscopic study with special reference to the islet organ of the splenic lobe. Anat. Rec. 178, 567–586 (1974)

    Google Scholar 

  • Trandaburu, T.: Comparative observations on adrenergic innervation and monoamine content in endocrine pancreas of some amphibians, reptiles and birds. Endokrinologie 59, 260–264 (1972)

    Google Scholar 

  • Vassallo, G., Solcia, E., Capella, C.: Light and electron microscopic identification of several types of endocrine cells in the gastrointestinal mucosa of the cat. Z. Zellforsch. 98, 333–356 (1969)

    Google Scholar 

  • Villamil, M.F.: Citogénesis del páncreas exo y endocrino en embriones de pollo. Rev. Soc. arg. Biol. 18, 416–424 (1942)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Andrew, A. Pancreatic APUD cells in older chick embryos, with special reference to their identity. Histochemistry 49, 213–219 (1976). https://doi.org/10.1007/BF00492376

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00492376

Keywords

Navigation