Summary
Pancreatic APUD cells showing formaldehyde-induced fluorescence in Black Australorp chick embryos of nine to eighteen days of incubation, proved, on subsequent staining and silver impregnation, to be A, B, D and, from sixteen days, enterochromaffin (EC) cells. EC and D cells were scattered in the exocrine parenchyma, the latter cells increasing with time. Some groups of B cells were associated with large A islets from the ninth day of incubation onwards. The composition of A islets (A and some D cells) and B islets (B and some D cells) and the distribution attained (A islets in the splenic and third lobes; B islets in all lobes), accords with the situation reported for adults.
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Andrew, A.: APUD cells in the endocrine pancreas and the intestine of chick embryos. Gen. comp. Endocr. 26, 485–495 (1975)
Andrew, A.: An experimental investigation into the possible neural crest origin of pancreatic APUD (islet) cells. J. Embryol. exp. Morph. 35, 577–593 (1976)
Cegrell, L.: The occurrence of biogenic amines in the mammalian endocrine pancreas. Acta physiol. scand., Suppl. 314, 40–41 (1968a)
Cegrell, L.: Uptake of L-Dopa in the pancreatic islets of albino mice. Acta physiol. scand., Suppl. 314, 35–39 (1968b)
Cegrell, L.: The occurrence of biogenic amines in the mammalian endocrine pancreas. Acta physiol. scand., Suppl. 314, 6–7 (1968c)
Cegrell, L., Falck, B., Rosengren, E.: Monoamines in the pig pancreas with special reference to the endocrine part. Acta physiol scand., Suppl. 314, 8–14 (1968)
Dieterlen-Lièvre, F.: Demonstration de l'activite precoce des cellules A du pancréas chez l'embryon de poulet. C.R. Acad. Sci. (Paris) 256, 1597–1599 (1963)
Dieterlen-Lièvre, F.: Etude morphologique et expérimentale de la différenciation du pancréas chez l'embryon de poulet. Bull. Biol. France Belg. 99, 3–116 (1965)
Dieterlen-Lièvre, F., Beaupain, D.: Etude immunocytologique de la différenciation du pancréas endocrine chez l'embryon de poulet. I. Îlots à insuline. Gen. comp. Endocr. 22, 62–69 (1974)
Enemar, A., Falck, B., Håkanson, R.: Observations on the appearance of norepinephrine in the sympathetic nervous system of the chick embryo. Develop. Biol. 11, 268–283 (1965)
Epple, A., Lewis, T.L.: Comparative histophysiology of the pancreatic islets. Amer. Zool. 13, 567–590 (1973)
Falck, B.: Observations on the possibilities of the cellular localization of monoamines by the fluorescence method. Acta physiol. scand. 56, Suppl. 197, 1–25 (1962)
Falck, B., Hellman, B.: Evidence for the presence of biogenic amines in pancreatic islets. Experientia (Basel) 19, 139–140 (1963)
Falck, B., Owman, C.: 5-Hydroxytryptamine and related amines in endocrine cell systems. Advanc. Pharmacol. 6, 211–231 (1968)
Hellerström, C., Hellman, B.: Some aspects of silver impregnation of the islets of Langerhans in the rat. Acta endocr. (Kbh.) 35, 518–532 (1960)
Hellman, B., Hellerström, C.: The islets of Langerhans in ducks and chickens with special reference to the argyrophil reaction. Z. Zellforsch. 52, 278–290 (1960)
Jennings, B.M.: Aldehyde-fuchsin staining applied to frozen sections for demonstrating pituitary and pancreatic beta cells. J. Histochem. Cytochem. 13, 328–333 (1965)
Legg, P.G.: Fluorescence studies on structures and endocrine cells in the pancreas of the cat. Z. Zellforsch. 88, 487–495 (1968)
Levene, C., Feng, P.: Critical staining of pancreatic alpha granules with phosphotungstic acid haematoxylin. Stain Technol. 39, 39–41 (1964)
Mikami, S.I., Mutoh, K.: Light and electron microscopic studies of the pancreatic islet cells in the chicken under normal and experimental conditions. Z. Zellforsch. 116, 205–227 (1971)
Mikami, S.I., Ono, K.: Glucagon deficiency induced by extirpation of alpha islets of the fowl pancreas. Endocrinol. 71, 464–473 (1962)
Owman, C., Håkanson, R., Sundler, F.: Occurrence and function of amines in endocrine cells producing polypeptide hormones. Fed. Proc. 32, 1785–1791 (1973)
Pearse, A.G.E.: Histochemistry. Theoretical and applied, p. 919. London: J.&A. Churchill 1960
Pearse, A.G.E.: Common cytochemical properties of cells producing polypeptide hormones with particular reference to calcitonin and the thyroid C cells. Vet. Rec. 79, 587–590 (1966)
Pearse, A.G.E., Polak, J.M., Heath, C.M.: Development, differentiation and derivation of the endocrine polypeptide cells of the mouse pancreas. Immunofluorescence, cytochemical and ultrastructural studies. Diabetologia 9, 120–129 (1973)
Pentila, A., Mustakallio, A.: Monoamine oxidase activity as related to the 5-hydroxytryptamine stores in the chicken duodenum during development. Acta histochem. (Jena) 31, 1–13 (1968)
Quickel, K.E., Feldman, J.M., Lebovitz, H.E.: Inhibition of insulin secretion by serotonin and dopamine: species variation. Endocrinology 89, 1295–1302 (1971)
Romanoff, A.L.: The avian embryo, p. 527. New York: Macmillan 1960
Sandstrom, R.H.: The differentiation of hepatic and pancreatic tissues of the chick in chorio-allantoic grafts. Physiol. Zool. 7, 226–246 (1934)
Slanina, P., Tjälve, H.: Uptake of 3H-atropine in pancreatic islets and the parafollicular cells of the thyroid in mice shown by micro-autoradiography. Acta physiol. scand. 84, 254–260 (1971)
Smith, P.H.: Pancreatic islets of the coturnix quail. A light and electron microscopic study with special reference to the islet organ of the splenic lobe. Anat. Rec. 178, 567–586 (1974)
Trandaburu, T.: Comparative observations on adrenergic innervation and monoamine content in endocrine pancreas of some amphibians, reptiles and birds. Endokrinologie 59, 260–264 (1972)
Vassallo, G., Solcia, E., Capella, C.: Light and electron microscopic identification of several types of endocrine cells in the gastrointestinal mucosa of the cat. Z. Zellforsch. 98, 333–356 (1969)
Villamil, M.F.: Citogénesis del páncreas exo y endocrino en embriones de pollo. Rev. Soc. arg. Biol. 18, 416–424 (1942)
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Andrew, A. Pancreatic APUD cells in older chick embryos, with special reference to their identity. Histochemistry 49, 213–219 (1976). https://doi.org/10.1007/BF00492376
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DOI: https://doi.org/10.1007/BF00492376