Summary
Using topo-optical staining reactions, the presence and molecular order of three structural components of outer segments of frog retina were studied. These components included (1) an acidic polysaccharide texture, (2) free aldehyde groups which arise during formalin fixation and (3) the oligosaccharide chains of rhodopsin. Quantitative measurements of the dye binding and birefringence effects arising from the individual structural components in rod outer segments were made. Results indicated that all three structural components had a rather well-defined orientation within the ROS.
The spherulites phagocytized from the apical ends of ROSs by the pigment epithelium also demonstrate preferred orientation of the three structural components investigated.
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Anderson, H.D., Fisher, K.S.: The photoreceptor of diurnal squirrels: outer segment structure, disk shedding and protein renewal. J. Ultrastruct. Res. 55, 119–141 (1976)
Ashbel, R., Seligman, A.M.: A new reagent for the histochemical demonstration of active carbonyl groups. A new method for staining ketonic steroids. Endocrinology 44, 565–583 (1949)
Basinger, S., Hoffman, R., Matthes, M.: Photoreceptor shedding is initiated by light in the frog retina. Science 194, 1074–1076 (1976)
Besharse, J.C., Hollyfield, J.S., Rayborn, M.E.: Photoreceptor outer segments: accelerated membrane renewal in rods after exposure to light. Science 196, 536–538 (1977)
Bownds, D., Gordon-Walker, A., Gaide-Huguenin, A.C., Robinson, W.: Characterization and analysis of frog photoreceptor membranes. J. Gen. Physiol. 58, 225–237 (1971)
Bridges, C.D.B.: Vitamin A and the role of the pigment epithelium during bleaching and regeneration of rhodopsin in the frog eye. Exp. Eye Res. 22, 435–455 (1976)
Brown, P.K.: Rhodopsin rotates in the visual receptor membrane. Nature [New Biol.] 236, 35–38 (1972)
Cone, R.A.: Rotationaldiffusion of rhodopsin in the visual receptor membrane. Nature [New Biol.] 236, 39–43 (1972)
Corless, J.M.: The apparent mass, composition and localization of complex polysaccharides in frog retinal rod outer segments. Submitted to Histochemistry (1978)
Denton, E.J.: The contribution of oriented photosensitive and other molecules to the absorption of whole retina. Proc. Roy. Soc. B. 150, 78–94 (1959)
Dilley, R.A., McConnel, D.G.: Alpha-tocopherol in the retinal outer segment of bovine eyes. J. Membrane Biol. 2, 317–323 (1970)
Dratz, E.A., Schwartz, S.: Where is rhodopsin? Nature [New Biol.] 242, 212–213 (1973)
Gould, E.S.: Mechanism and Structure in Organic Chemistry, p. 708. New York: Holt, Rinehart and Winston 1969
Hall, M.O., Hall, D.O.: Superoxide dismutase of bovine and frog outer segments. Biochem. Biophys. Res. Comm. 67, 1199–1204 (1975)
Hayat, M.A.: Principles and Techniques of Electron Microscopy, Vol. 1, p. 84–87. New York: Van Nostrand Reinhold Co. 1970
Heller, J., Lawrence, M.A.: Structure of the glycopeptide from bovine visual pigment 500. Biochemistry 9, 864–869 (1970)
Jan, L.Y., Revel, J.-P.: Ultrastructural localization of rhodopsin in the vertebrate retina. J. Cell Biol. 62, 257–273 (1974)
LaVail, M.M.: Rod outer segment disk shedding in a retina: Relationship to cyclic lighting. Science 194, 1071–1074 (1976)
Liebman, P.A.: In situ microspectrophotometric studies on the pigments of single retinal rods. Biophys. J. 2, 161–178 (1962)
Lillie, R.D.: Histochemical studies on the retina. Anat. Rec. 112, 477–495 (1953a)
Lillie, R.D.: Ethylenic reaction of ceroid with performic acid Schiff reagent. Stain Technol. 27, 37–45 (1952b)
Lolley, R.N., Hess, H.H.: The retinal rod outer segment of the frog: Detachment, isolation, phosphorous fractions and enzyme activity. J. Cell Physiol. 73, 9–24 (1969)
Meier-Ruge, W.: Medikamentose Retinopathie. Stuttgart: George Thieme 1967
Millonig, G., Marinozzi, V.: Fixation and embedding in electron microscopy. Adv. Opt. Elect. Microscopy 2, 251–341 (1968)
Moody, M.F., Robertson, J.D.: The fine structure of some retinal photoreceptors. J. Biophys. Biochem. Cytol. 7, 87–91 (1960)
Pearce, A.G.E.: Histochemistry, Theoretical and Applied. V.1. London: Churchill Ltd. 1968
Robertson, J.D.: Granulo-fibrillar and globular substructure in unit membranes. Ann. New York Acad. Sci. 137, 421–440 (1966)
Romhányi, Gy.: Über die submikroskopische strukturelle Grundlage der metachromatischen Reaktion. Acta histochem. (Jena) 15, 201–233 (1963)
Romhányi, Gy., Deák, Gy., Fischer, J.: Aldehyde bisulfite-toluidine blue (ABT) staining as a topooptical reaction for the demonstration of linear order of vicinal OH groups in biological structures. Histochemistry 43, 333–348 (1975)
Romhányi, Gy., Molnár, L.: Optical polarisation indicates linear arrangement of rhodopsin oligosaccharide chain in rod disk membranes of frog retina. Nature (London) 249, 486–488 (1974)
Röhlich, P.: Photoreceptor membrane carbohydrate on the intradiscal surface of retinal rod disks. Nature (London) 263, 789–791 (1976)
Schmidt, W.J.: Doppelbrechung, Dichroismus und Feinbau des Außengliedes der Sehzellen von Frosch. Z. Zellforsch. 22, 485–498 (1935)
Sidman, R.L., Wislocki, G.B.: Histochemical observations on rods and cones in retinas of vertebrates. J. Histochem. Cytochem. 2, 413–433 (1954)
Sjöstrand, F.S.: Ultrastructure of retinal rods and cones. J. Cell. Comp. Physiol. 42, 33 (1953)
Sjöstrand, F.S.: Electron microscopy of retina. In: The Structure of the Eye (Ed. Smelser, G.K.), p. 1–26 New York: Academic Press 1961
Wald, G.: Molecular basis of visual excitation. Science 162, 230–239 (1968)
Walker, J.F.: Formaldehyde, 3rd Edition. New York: Reinhold Pub. Corp. 1964.
Weale, R.A.: Rod birefringence in light. Vision Res. 11, 1387–1393 (1971)
Wislocki, G.B., Sidman, R.L.: The chemical morphology of the retina. J. Comp. Neurol. 101, 53–99 (1954)
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Romhányi, G., Fischer, J. & Corless, J.M. Orientation of acidic polysaccharides and rhodopsin-oligosaccharides in frog retinal rod outer segments. Histochemistry 56, 65–77 (1978). https://doi.org/10.1007/BF00492254
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DOI: https://doi.org/10.1007/BF00492254