Summary
Acid phosphatase, alkaline phosphatase, glucose-6-phosphatase, Mg-activated adenosine triphosphatase and 5′ nucleotidase were demonstrated in the rat liver using a cerium-based method. This method can be applied routinely and yields better results than the lead-based method. The tissue was postfixed in osmium tetroxide and potassium ferrocyanide which considerably enhances the membrane contranst in comparison with solely osmium tetroxide postfixation. This facilitates the precize localization of the reaction product.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barka T, Anderson PJ (1962) Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler. J Histochem Cytochem 10:741–753
Blok J, Onderwater JJM, de Water R, Ginsel LA (1982) A cytochemical method for the demonstration of 5′ nucleotidase in mouse peritoneal macrophages, with cerium ions used as trapping agent. Histochemistry 75:437–443
Briggs RT, Draht DB, Karnovsky ML, Karnovsky MJ (1975) Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. J Cell Biol 67:566–586
De Bruyn WC (1968) A modified OsO4-(double) fixation procedure which selectively contrasts glycogen. In: Bocciarelli DS (ed) Electron microscopy 1968, Vol. II. Tipografia Poliglotta Vaticana, Roma, pp 65–66
De Bruyn WC, Van Buitenen JMH (1980) X-ray microanalysis of aldehyde-fixed glycogen contrast-stained by OsVI·FeII and OsVI·RuIV complexes. J Histochem Cytochem 28:1242–1250
Hugon J, Borgers MA (1966) A direct lead method for the electron microscopic visualization of alkaline phosphatase activity. J Histochem Cytochem 14:429–431
Hugon J, Borgers M, Maestracci D (1970) Glucose-6-phosphatase and thiamine pyrophosphatase activities in the jejunal epithelium of the mouse. J Histochem Cytochem 18:361–364
Karnovsky MJ (1971) Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. J Cell Biol 51:146A
Riemersma JC (1970) Chemical effects of fixation on biological specimens. In: Parsons DF (ed) Some biological techniques in electron microscopy. Academic Press, New York, pp 69–99
Robinson JM, Karnovsky MJ (1982) Cytochemical localization of the ectoenzyme 5′-nucleotidase by a new cytochemical method. J Histochem Cytochem 30:575
Veenhuis M, Van Dijken JP, Harder W (1980a) A new method for the cytochemical demonstration of phosphatase activities in yeasts based on the use of cerous ions. FEMS Microbiol Lett 9:285–291
Veenhuis M, Flik G, Wendelaar Bonga SE (1980b) CeCl3: a new capturing agent for the cytochemical demonstration of phosphatase activity. In: Brederoo P, De Priester W (eds) Electron microscopy 1980, Vol. 2. Seventh European Congress on Electron Microscopy Foundation, Leiden, pp 284–285
Wachstein M, Meisel E (1957) Histochemistry of hepatic phosphatases at a physiologic pH. With special reference to the demonstration of bile canaliculi. Am J Clin Pathol 27:13–23
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hulstaert, C.E., Kalicharan, D. & Hardonk, M.J. Cytochemical demonstration of phosphatases in the rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation. Histochemistry 78, 71–79 (1983). https://doi.org/10.1007/BF00491113
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00491113