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Microphotometry of rat liver nucleoproteins during the cell cycle, and comparison of diploid nuclei in the G2 period with tetraploid nuclei

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Summary

The following facts were established with a microphotometric investigation of isolated nucleic from rat liver in different stages of the cell cycle.

During the mitotic wave occurring in the liver of newborn animals after injection of casein it was found that the naphtol yellow S (NYS) protein content of the nuclei increases about 30% during the G1-period. A second increase of around 70% was established during the S-phase whereas no increase could be observed during the G2-phase. An indication for the existence of a “crital protein mass” of the nuclei before the onset of the S-phase could be observed.

The protein content of diploid nuclei in the G1-phase of adult animals is about 50% higher than in newborns. This makes it probable that there is no significant difference in the NYS-protein content of diploid nuclei in the G2-period and tetraploid nuclei of adult rats. No differences were observed between diploid nuclei in the G2-period of newborn rats and tetraploid nuclei of adult rats in their Fastgreen histon, RNA and SH plus SS content.

The only criterion to distinguish between G2 nuclei and tetraploid nuclei seems to be the number of nucleoti, but this is rather unreliable.

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References

  • Barnett, R. J., Seligman, A. M.: Histochemical demonstration of sulphydryl and disulfide groups of protein. J. nat. Cancer Inst. 14, 769–792 (1954)

    Google Scholar 

  • Bloch, D. P., Goodman, G. V.: A microphotometric study of the syntheses of desoxyribonucleic acid and nuclear histone. J. biophys. biochem. Cytol. 1, 1–28 (1955)

    Google Scholar 

  • Incefy, S. S., Kappas, A.: Isolation and biochemical characterization of nuclei from chick embryo liver. J. Cell Biol. 50, 385–398 (1971)

    Google Scholar 

  • Kiefer, G., Kiefer, R., Sandritter, W.: Cytophotometric determination of nucleic acids in U.V. light and after gallocyanin chromalum staining. Exp. Cell Res. 45, 247–249 (1966)

    Google Scholar 

  • Meinders-Groeneveld, J., James, J.: Some quantitative data regarding the nucleoli in cell nuclei from rat liver of different ploidy classes. Z. Zellforsch. 114, 165–174 (1971)

    Google Scholar 

  • Mitchison, J. M.: The biology of the cell cycle, p. 88. Cambridge University Press 1971

  • Monesi, V.: DNA, RNA and protein syntheses during the mitotic cell cycle. In: Handbook of molecular cytology, A. Lima de Faria, p. 473–495. Amsterdam-London: North Holland Publ. Comp. 1969

    Google Scholar 

  • Morselt, A. F. W., Braakman, D. J., James, J.: Feulgen-DNA and Fastgreen histone estimations in individual cell nuclei. Acta histochem. (Jena) 43, 381–286 (1972)

    Google Scholar 

  • Morselt, A. F. W., Maarschalkerweerd, V.: Microphotometry of rat liver nucleoproteins. Histochemistry 41, 107–110 (1974)

    Google Scholar 

  • Nadal, C.: Synchronization of baby rat hepatocytes a new test for the detection of factors controlling DNA synthesis in rat hepatic cells. Cell Tiss. Kin. 6, 473–446 (1973)

    Google Scholar 

  • Robbins, E., Borum, T. W.: The cytoplasmic synthesis of histones in Hela cells and its temporal relationship to DNA replication. Proc. nat. Acad. Sci. (Wash.) 57, 409 (1967)

    Google Scholar 

  • Schiemer, A. G.: Über Schnellphotometrie mit dem Zeisschen Universal Mikrospektro-photometer (UMSP1). Z. wiss. Mikr. 69, 244–256 (1969)

    Google Scholar 

  • Sillevis Smitt, P. A. E., James, J., Wisse, J. H.: The DDD-method in the cytophotometric quantitative estimation of protein bound sulphydril groups in palatal smears. Acta histochem. (Jena) 33, 53–58 (1969)

    Google Scholar 

  • Tas, J., Oud, P. S., James, J.: The naphtol yellow-S stain for proteins tested in a model system of polyacrylamide films and evaluated for practical use in histochemistry. Histochemistry 40, 231–240 (1974)

    Google Scholar 

  • Zetterberg, A.: Protein migration between cytoplasm and cell nucleus during interphase in mouse fibroblast in vitro. Exp. Cell Res. 43, 526–536 (1966)

    Google Scholar 

  • Zetterberg, A., Killander, D.: Quantitative cytophotometric and autoradiographic studies on the rate of protein synthesis during interphase in mouse fibroblasts in vitro. Exp. Cell Res. 40, 1–11 (1965)

    Google Scholar 

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Authors and Affiliations

  1. Histological Laboratory, University of Amsterdam, Amsterdam, The Netherlands

    Antonius F. W. Morselt & Hannie G. M. van Wijgerden

  2. Histologisch Laboratorium, Jan Swammerdam Institut, 1e Constantijn Huygensstraat 20, Amsterdam, The Netherlands

    Antonius F. W. Morselt

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  1. Antonius F. W. Morselt
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  2. Hannie G. M. van Wijgerden
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Morselt, A.F.W., van Wijgerden, H.G.M. Microphotometry of rat liver nucleoproteins during the cell cycle, and comparison of diploid nuclei in the G2 period with tetraploid nuclei. Histochemistry 44, 87–93 (1975). https://doi.org/10.1007/BF00490423

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  • DOI: https://doi.org/10.1007/BF00490423

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