Summary
Distribution and amount of neuropeptide Y- and synaptophysin-immunoreactive nervous structures within the heart were investigated in dogs 4 days after ligation of the left anterior descending coronary artery (LAD). In the right atrium and posterior left ventricular regions, which were taken as (non-infarcted) control areas, neuropeptide Y-immunoreactive paravascular nerves and a perivascular nerve plexus running within the adventitia of the coronary arteries and their branches down to the arterioles were observed. Morphometric measurements of the area density revealed 0.099±0.014% for synaptophysin- and 0.037±0.0072% for neuropeptide Y-immunoreactivity within the posterior wall of the left ventricular myocardium. Four days after ligation of the LAD only single synaptophysin-and neuropeptide Y-immunoreactive nerve fibers were very rarely detected in the infarcted region of the anterior wall of the left ventricle. Above the ligature larger than normal neuropeptide Y-immunoreactive axons within nerves along the LAD indicated a blockage of the axoplasmic transport of this peptide.
When investigating this model of experimental myocardial infarction, mechanical traumatization of peri- and paravascular nerves of the LAD by the ligature has to be considered as a major pathogenetic factor, in addition to ischemia leading to denervation of infarcted as well as nonischemic myocardium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aidonidis I, Metz J, Gerstheimer F, Trautner H, Herbst M, Brachmann J (1986) Electrophysiologic and immunocytochemical observations in a coronary artery ligation and intramural occlusion model of recent myocardial infarction. Circulation 74:II-252, 1053
Borchard F, Paessens R (1980) Morphology of cardial nerves in experimental infarction of rat. II. Electron microscopic findings. Virchows Arch A Pathol Anat and Histol 386:279–291
Chilian WM, Boatwright RB, Shoji T, Griggs DM (1982) Regional uptake of H3-norepinephrine by the canine left ventricle. Proc Soc Exp Biol Med 171:158–163
Dahlström A, Fuxe K, Mya-Tu M, Zetterström BEM (1965) Observations on adrenergic innervation of dog heart. Am J Physiol 209:689–692
Dolezel S, Gerova M, Gero J, Sladek T, Vasku J (1978) Adrenergic innervation of the coronary arteries and the myocardium. Acta Anat 100:306–316
Dolezel S, Gerova M, Hartmannova B, Dostal M, Janeckova H, Vasku J (1985) Cardiac adrenergic innervation after instrumentation of the coronary artery in dog. Am J Physiol 246:H459-H465
Ekblad E, Edvinsson L, Wahlestedt C, Uddan R, Hankanson R, Sundler F (1984) Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers. Reg Peptides 8:225–235
Gerstheimer FP, Simon T, Kölb J, Höpker W, Metz J (1988) Computer-assisted morphometric study of the innervation of the guinea pig heart. Histochemistry 88:545–551
Gu J, Polak JM, Allen JM, Huang WM, Sheppard MN, Tatemoto K, Bloom SR (1984) High concentrations of a novel peptide, neuropeptide Y, in the innervation of mouse and rat heart. J Histochem Cytochem 32:467–472
Hirche H, McDonald F, Polwin W, Addicks K (1985) Vicious cycle of catecholamines and K+ in cardiac ischemia. J Cardiovasc Pharmacol 7:71–75
Holmgren S, Abrahamsson T, Almgren O, Eriksson BM (1981) Effect of ischemia on the adrenergic neurons of the rat heart: a fluorescence histochemical and biochemical study. Cardiovasc Res 15:680–689
Holmgren S, Abrahamsson T, Almgren O (1985) Adrenergic innervation of coronary arteries and ventricular myocardium in the pig: fluorescence microscopic appearance in the normal state and after ischemia. Basic Res Cardiol 80:18–26
Lundberg JM, Terenius L, Hoekfelt T, Martling CR, Tatemoto K, Mutt V, Polak J, Bloom S, Goldstein M (1982) Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand 116:477–480
Malmfors T, Sachs C (1965) Direct studies on the disappearance of the transmitter and changes in the uptake-storage mechanisms of degenerating adrenergic nerves. Acta Physiol Scand 64:211–223
Mathes P, Gudbjarnason S (1971) Changes in norepinephrine stores in the canine heart following experimental myocardial infarction. Am Heart J 81:211–219
Mayor D, Kapeller K (1967) Fluorescence microscopy and electron microscopy of adrenergic nerves after constriction at two points. J Microsc (London) 87:277–294
Metz J, Gerstheimer F, Herbst M (1987a) Synaptophysin, a neuronal marker in the guinea pig heart. Histochemistry 86:221–224
Metz J, Gerstheimer FP, Höpker W (1987b) Quantitative Immunhistochemie endokriner Zellen. Labor-Medizin 10:3–9
Metz J, Gerstheimer F, Trautner H, Aidonidis I, Brachmann J (1987c) Herzinnervation nach koronarer Okklusion: morphologische Analyse. Kardiologie (abstr.) 76:185
Metz J, Gerstheimer F, Trautner H, Aidonidis I, Brachmann J, Kübler W (1989) Heart innervation after transluminal occlusion of the left anterior descending coronary artery (LAD). Cell Tissue Res (in press)
Navone F, Jahn R, Di Gioia G, Stutenbrok H, Greengard H, De Camilli P (1986) Protein p 38: An intergral membrane protein specific for small vesicles of neurons and neuroendocrine cells. J Cell Biol 103:2511–2527
Paessens R, Borchard F (1980) Morphology of cardiac nerves in experimental infarction of rat hearts. I. Fluorescence microscopy findings. Virchows Archiv A Pathol Anat Histopathol 386:265–278
Schömig A, Dart AM, Dietz R, Mayer E, Kübler W (1984) Release of endogenous catecholamines in the ischemic myocardium of the cat. Part A: Locally mediated release. Circ Res 55:689–701
Schömig A, Fischer S, Kurz T, Richardt G, Schömig E (1987) Non-exocytotic release of endogenous noradrenaline in the ischemic and anoxic rat heart: mechanism and metabolic requirements. Circ Res 60:194–205
Sternberger LA (1979) Immunocytochemistry, 2nd edn. J. Wiley, New York
Trump BF, Berezesky K, Collan Y, Kahn MW, Mergner WJ (1976) Recent studies on the pathophysiology of ischemic cell injury. Beitr Pathol 158:363–388
Weihe E, Reinecke M, Forssmann WG (1984) Distribution of vasoactive intestinal polypeptide-like immunoreactivity in the mammalian heart. Cell Tissue Res 236:527–540
Wiedenmann B, Franke W (1985) Identification and localization of synaptophysin, an intergral membrane glycoprotein of MW 38.000, characteristic of presynaptic vesicles. Cell 41:1017–1028
Author information
Authors and Affiliations
Additional information
This study was supported by the SFB 320 within the Deutsche Forschungsgemeinschaft (DFG)
Rights and permissions
About this article
Cite this article
Trautner, H., Gerstheimer, F., Aidonidis, I. et al. Heart innervation after ligation of the left anterior descending coronary artery (LAD). Histochemistry 92, 103–108 (1989). https://doi.org/10.1007/BF00490227
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00490227