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The value of hyaluronidase treatment of different tissues before demonstration of fibronectin by the indirect immunoperoxidase technique

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Summary

The influence of testicular hyaluronidase treatment on the immunohistochemical localization of fibronectin in different tissues (human articular cartilage, large intestine, synovial membrane and experimental granulation tissue) as well on frozen as on formaldehyde fixed, paraffin embedded tissue, has been studied using the indirect immunoperoxidase technique. Pretreatment with hyaluronidase is essential in demonstrating fibronectin in frozen sections of human articular cartilage. In the other tissues examined treatment with hyaluronidase was not essential, but gave a more optimal staining quality. The effect of hyaluronidase treatment was to some extent dependent on the duration of treatment. In formaldehyde fixed, paraffin processed tissue the improvement with hyaluronidase treatment was only seen when the hyaluronidase followed pepsin digestion of the deparaffinized tissue sections.

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References

  • Bayliss MT, Ali SY (1978) Age-related changes in the composition and structure of human articular-cartilage proteoglycans. Biochem J 176:683–693

    Google Scholar 

  • Brozman M (1978) Immunohistochemical analysis of formaldehyde- and trypsin- or pepsintreated material. Acta Histochem 63:251–260

    Google Scholar 

  • Burns J, Dixon AJ, Woods JC (1980) Immunoperoxidase localization of fibronectin in glomeruli of formalin fixed paraffin processed renal tissue. Histochemistry 67:73–78

    Google Scholar 

  • Clausen PP, Thomsen P (1978) Demonstration of hepatitis B-surface antigen in liver biopsies. Acta Pathol Microbiol A 86:383–388

    Google Scholar 

  • Clemmensen I Andersen RB (1982) Different molecular forms of fibronectin in rheumatoid synovial fluid. Arthritis Rheum 25:25–31

    Google Scholar 

  • Clemmensen I, Hølund B, Andersen RB (1982) Fibrin and fibronectin in rheumatoid synovial membrane and rheumatoid synovial fluid. Submitted — Arthritis Rheum

  • Clemmensen I, Hølund B, Johansen N, Andersen RB (1982) Demonstration of fibronectin in human articular cartilage by an indirect immunoperoxidase technique. Histochemistry 76:51–56

    Google Scholar 

  • Cooper SM, Keyser AJ, Beaulieu AD, Rouslahti E, Nimni ME, Quismorie FB (1979) Increase in fibronectin in the deep dermis of involved skin in progressive systemic sclerosis. Arthritis Rheum 22:983–987

    Google Scholar 

  • Couchman JR, Gibson WT, Thom D, Weaver AC, Rees DA, Parish WE (1979) Fibronectin distribution in epithelial and associated tissue in rat. Arch Dermatol Res 266:295–310

    Google Scholar 

  • Dessau W, Sasse J, Timpl R, Jilek F, Mark K von der (1978) Synthesis and extracellular deposition of fibronectin in chondrocyte culture. J Cell Biol 79:342–355

    Google Scholar 

  • Dixon AJ, Burns J, Dunnill MS, McGee J (1980) Distribution of fibronection in normal and diseased human kidneys. J Clin Pathol 33:1021–1028

    Google Scholar 

  • Engvall E, Rouslahti E (1977) Binding of soluble form of fibroblast surface protein, fibronectin to collagen. Int J Cancer 20:1–5

    Google Scholar 

  • Engvall E, Rouslahti E, Miller EJ (1978) Affinity of fibronectin to collagen of different genetic types and to fibrinogen. J Exp Med 147:1584–1595

    Google Scholar 

  • Fyrand O (1979) Studies of fibronectin in the skin. I. Indirect immunofluorescence studies in the normal human skin. Br J Dermatol 101:263–270

    Google Scholar 

  • Hahn E, Wick G, Pencer D, Timpl R (1980) Distribution of basement membrane proteins in normal and fibrotic human liver: Collagen type IV, laminin and fibronectin. Gut 21:63–71

    Google Scholar 

  • Hølund B, Junker P, Garbarsch C, Christoffersen P, Lorenzen I (1979) Formation of granulation tissue in subcutaneously implanted sponges in rats. Acta Pathol Microbiol Scand A 87:367–374

    Google Scholar 

  • Hølund B, Clausen PP, Clemmensen I (1981) The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry 72:291–299

    Google Scholar 

  • Hølund B, Clemmensen I, Junker P, Lyon H (1982) Fibronectin in experimental granulation tissue. Acta Pathol Microbiol Immunol Scand A 90:159–165

    Google Scholar 

  • Jilek K, Hörmann H (1978) Cold-insoluble globulin (fibronectin). Affinity to soluble collagen of various types. Hoppe-Seyler's Physiol Chem 359:247–250

    Google Scholar 

  • Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51

    Google Scholar 

  • Laterra J, Culp LA (1982) Differences in hyaluronate binding to plasma and cell surface fibronectins. J Biol Chem 257:719–726

    Google Scholar 

  • Linder E, Stenman S, Lehto V-P, Vaheri A (1978) Distribution of fibronectin in human tissue and relationship to other connective tissue components. Ann NY Acad Sci 312:151–159

    Google Scholar 

  • Mosesson MW (1978) Structure of human plasma cold-insoluble globulin and the mechanism of its precipitation in the cold with heparin or fibrin—fibrinogen complexes. Ann NY Acad Sci 312:11–30

    Google Scholar 

  • Mosesson MW, Amrani DL (1980) The structure and biologic activities of plasma fibronectin. Blood 56:145–158

    Google Scholar 

  • Pearlstein E, Dienstman XR, Defendi V (1978) Identification of macrophage external membrane proteins and their possible role in cell adhesion. J Cell Biol 79:263–267

    Google Scholar 

  • Pettersson EE, Colvin RB (1978) Cold-insoluble globulin (fibronectin, LETs protein) in normal and diseased human glomeruli. Clin Immunol Immunopathol 11:425–436

    Google Scholar 

  • Repech LA, Fitzgerald TJ, Furcht LT (1982) Fibronectin involvement in granulation tissue and wound healing in rabbits. J Histochem Cytochem 30:351–358

    Google Scholar 

  • Ruoslahti E, Pierschbacher M, Hayman EG, Engvall E (1982) Fibronectin: a molecule with remarkable structural and functional diversity. TIBS 188-190

  • Scott D, Delamere JP, Walton KW (1981) The distribution of fibronectin in the pannus in rheumatoid arthritis. Br J Exp Pathol 62:362–368

    Google Scholar 

  • Stenamn S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal human tissue. J Exp Med 147:1054–1064

    Google Scholar 

  • Weiss, MA, Ooi BS, Ooi YM, Engvall E, Rouslahti E (1979) Immunofluorescent localization of fibronectin in the human kidney. Lab Invest 41:340–347

    Google Scholar 

  • Weiss RE, Reddi AH (1981) Appearance of fibronectin during the differentiation of cartilage, bone and bone marrow. J Cell Biol 88:630–636

    Google Scholar 

  • Yamada KM, Yamada SS, Pastan I (1976) Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts. Proc Natl Acad Sci USA 73:1217

    Google Scholar 

  • Yamada KM, Olden K (1978) Fibronectin adhesive glycoprotein of cell surface and blood. Nature 275:179–185

    Google Scholar 

  • Yamada KM, Kennedy DW, Kimata K, Pratt RM (1980) Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. J Biol Chem 255:6055–6063

    Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Chemistry, Hvidovre Hospital, University of Copenhagen, Denmark

    B. Hølund & I. Clemmensen

  2. Department of Pathological Anatomy, Hvidovre Hospital, University of Copenhagen, DK-2650, Hvidovre, Denmark

    B. Hølund & I. Clemmensen

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  1. B. Hølund
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  2. I. Clemmensen
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Hølund, B., Clemmensen, I. The value of hyaluronidase treatment of different tissues before demonstration of fibronectin by the indirect immunoperoxidase technique. Histochemistry 76, 517–525 (1982). https://doi.org/10.1007/BF00489906

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  • DOI: https://doi.org/10.1007/BF00489906

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