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Histochemistry

, Volume 48, Issue 1, pp 17–27 | Cite as

Rapid microspectrofluorometric studies in EL2 cells following intracellular accumulation of dibenzocarbazoles

  • Michel Deumie
  • Elli Kohen
  • Pierre Viallet
  • Cahide Kohen
  • Jean-Marie Salmon
Article

Summary

Microspectrofluorometric observations were carried out in EL2 ascites cancer cells and dibenzo(a,e)fluoranthene (diB(a,e)F)-grown EL2 cells, following treatment (5 min) with three dibenzocarbazoles (1,2,7,8; 1,2,5,6 and 3,4,5,6). After microinjection of glucose-6-P leading to reduction of NAD(P), a sequence of difference spectra (after substrate minus before) is recorded. In dibenzocarbazole-untreated cells, maximum NAD(P) reduction (emission maximum at 465–475 nm) is attained within 5 s, followed by a gradual return to initial fluorescence within 20 to 200 s (faster in the diB(a,e)F-grown). In dibenzocarbazole-treated cells there is a rather regular increase in the intensity of the difference spectrum up to ∼ 300–500 s. Initially the increase is more predominant in the region around 460–470 nm, but it gains later prominence in the shorter wavelength region (420–430 nm) characteristic of the hydrocarbon (higher and steadier increase in the 3,4,5,6, dibenzocarbazole-treated diB(a,e)F-grown). Subsequently there is a gradual decrease of fluorescence which may or may not return to initial level. The observed increase spectra require evaluation in terms of possible components (e.g. a mixture of NAD(P)H and hydrocarbon, binding changes, succession of fluorescent metabolites).

Keywords

Hydrocarbon Short Wavelength Fluoranthene Emission Maximum Steady Increase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Michel Deumie
    • 1
  • Elli Kohen
    • 1
  • Pierre Viallet
    • 1
  • Cahide Kohen
    • 1
  • Jean-Marie Salmon
    • 1
  1. 1.Papanicolaou Cancer Research InstituteMiamiUSA

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