Skip to main content
SpringerLink
Log in

Genetic control of alcohol dehydrogenase isozymes in maize

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

By means of horizontal gel electrophoresis and the zymogram technique, genetic variants and the formation of a hybrid molecule of the enzyme alcohol dehydrogenase (ADH) have been found in Zea mays. Each inbred homozygous stock examined showed two types of ADH isozyme patterns: a fast faint zone and a slower deeply staining zone, both anode-migrating at pH 8.5. The variants found differed in that each of the ADH zones varied in its electrophoretic mobility when compared to its counterpart in the other strain. When appropriate genetic crosses were made, the resulting heterozygotes showed the parental ADH zones, and, in addition, a band of intermediate mobility was formed between the deep-staining ADH bands. However, in the fast-moving zone only the parental isozymes were represented in the heterozygote. The formation of the hybrid molecule and the apparent gene dosage effects support the hypothesis that ADH-2 in maize exists as a dimer, whereas ADH-1 may exist as a monomer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beckman, L., Scandalios, J.G., and Brewbaker, J.L. (1964). Genetics of leucine aminopeptidase isozymes in maize. Genetics 50 899.

    Google Scholar 

  • Poulik, M. D. (1957). Starch gel electrophoresis in a discontinuous system of buffers. Nature 180 1477.

    Google Scholar 

  • Scandalios, J.G. (1965). Subunit dissociation and recombination of catalase isozymes. Proc. Nat. Acad. Sci., Wash. 53 1305.

    Google Scholar 

  • Scandalios, J. G. (1966a). Amylase isozyme polymorphism in maize. Planta (Berlin) 69 244.

    Google Scholar 

  • Scandalios, J. G. (1966b). Genetic variation of alcohol dehydrogenase in maize. (Abstr.) Genetics 54 359.

    Google Scholar 

  • Scandalios, J. G. (1967). Human serum leucine aminopeptidase: Variation in pregnancy and in disease states. J. Hered. (in press).

  • Schwartz, D. (1960). Genetic studies on mutant enzymes in maize: Synthesis of hybrid enzymes by heterozygotes. Proc. Nat. Acad. Sci., Wash. 46 1210.

    Google Scholar 

  • Schwartz, D., and Endo, T. (1966). Alcohol dehydrogenase polymorphism in maize—Simple and compound loci. Genetics 53 709–715.

    Google Scholar 

  • Shaw, C. R. (1965). Electrophoretic variation in enzymes. Science 149 936.

    Google Scholar 

  • Ursprung, H., and Leone, J. (1965). Alcohol dehydrogenase: A polymorphism in Drosophila melanogaster. J. Exp. Zool. 160 147.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MSU/AEC Plant Research Laboratory, Michigan State University, East Lansing, Michigan

    John G. Scandalios

Authors
  1. John G. Scandalios
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the U.S. Atomic Energy Commission, under contract No. AT(11-1)-1338.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scandalios, J.G. Genetic control of alcohol dehydrogenase isozymes in maize. Biochem Genet 1, 1–9 (1967). https://doi.org/10.1007/BF00487731

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00487731

Keywords

Search

Navigation