Abstract
A microscale isoelectric focusing technique has been developed and used to study hypoxanthine-guanine phosphoribosyl transferase (HGPRT; E.C. 2.4.2.8, inosinate-guanylate:pyrophosphate phosphoribosyl transferase) activities in mouse and human cell lines. The enzymes of both mouse and human origin are shown to exhibit considerable heterogeneity, but each type has a unique range of isoelectric pH. The enzyme of a mouse × human hybrid cell line, derived by fusion of HGPRT− parental cells, gives a homogeneous peak of activity, unlike the wild-type enzyme of either parent. The possibility is suggested that this enzyme activity is due to intra-allelic complementation.
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Centennial Fellow of the Medical Research Council of Canada, 1967–1970.
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Roy, K.L., Ruddle, F.H. Microscale isoelectric focusing studies of mouse and human hypoxanthine-guanine phosphoribosyl transferases. Biochem Genet 9, 175–185 (1973). https://doi.org/10.1007/BF00487447
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DOI: https://doi.org/10.1007/BF00487447