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Genetic control of leucine aminopeptidase and esterase isozymes in the interspecific cross Cucurbita ecuadorensis x C. maxima

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Abstract

Starch gel electrophoretic analyses of crude seed extracts of Cucurbita ecuadorensis, C. maxima, their F1 and F2, and three of the four possible interspecific backcrosses reveal that the genus is polymorphic for alpha-naphthyl acetate esterases (Est) and leucine aminopeptidase (LAP). The two electrophoretic forms of both Est and LAP are controlled by codominant alleles. The two loci do not exhibit linkage. Neither the LAP nor the Est phenotypes exhibit a significant deviation from the expected 1:1 ratio in interspecific backcrosses when the donor parent alleles are transmitted through female gametes, but there is a significant deviation for Est when transmission is through male gametes. Differential gametic selection involving the Est-1 locus suggests structural differences between the genomes of the parental species for the chromosomal region in which this locus occurs. No structural differences are indicated between the parental genomes for the chromosome region bearing the Lap-1 locus.

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Authors and Affiliations

  1. Department of Biology, George Mason College of the University of Virginia, Fairfax, Virginia

    J. R. Wall

  2. U.S. Department of Agriculture, ARS, Crops Research, La Jolla, California

    Thomas W. Whitaker

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  1. J. R. Wall
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  2. Thomas W. Whitaker
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Wall, J.R., Whitaker, T.W. Genetic control of leucine aminopeptidase and esterase isozymes in the interspecific cross Cucurbita ecuadorensis x C. maxima . Biochem Genet 5, 223–229 (1971). https://doi.org/10.1007/BF00485793

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  • DOI: https://doi.org/10.1007/BF00485793

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