Abstract
Four anionic peroxidase isoenzymes in main stem tissues of flax (Linum usitatissimum L.) were separated electrophoretically on acrylamide gels and their individual activities measured spectrophotometrically in the gels. Activities were expressed in terms of areas of optical density peaks corresponding to the locations of the isoenzymes. Four isoenzymes were assayed for activity in this way in two inbred genotypes and their F1, F2, and first backcross progeny. Crosses were made in all combinations between the two parental genotypes and their reciprocal F1 hybrids to produce 16 progenies of the generations above. Isoenzyme separations and assays for activity were carried out on individual plants of each of the 16 progenies. A model estimating cytoplasmic effects across segregating generations as differences between all progeny of the one compared to the other F1 reciprocal hybrid, either in a male or a female direction, was fitted to the activity data by weighted least-squares procedures. Cytoplasmic effects transmitted through male gametes from the F1 reciprocals were demonstrable for three of the four anionic peroxidase isoenzymes.
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Supported in part by the National Research Council of Canada.
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Tyson, H. Cytoplasmic effects on activity of individual anionic isoenzymes of peroxidase in crosses between two genotypes of flax (Linum usitatissimum L.). Biochem Genet 10, 13–21 (1973). https://doi.org/10.1007/BF00485744
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DOI: https://doi.org/10.1007/BF00485744