Abstract
Extracts from an acid phosphatase CRM− null mutant of Drosophila melanogaster were used to eliminate contaminating antibodies in a nonspecific preparation of anti-acid phosphatase serum. This method of producing specific antisera makes unnecessary the rigorous purification of an antigen prior to immunization attempts in those cases where CRM− null mutants of the antigen are available. Antisera so prepared could be used for a wide variety of purposes.
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Supported by NSF Grant BMS 72-02398 A02 (to N. A.).
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Arnheim, N., MacIntyre, R. Preparation of specific antisera to Drosophila acid phosphatase without rigorous protein purification. Biochem Genet 14, 237–243 (1976). https://doi.org/10.1007/BF00484763
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DOI: https://doi.org/10.1007/BF00484763