Abstract
The pyridine nucleotide metabolism of imaginal discs of Drosophila melanogaster has been studied in vitro by incubating discs with labeled nicotinic acid in the presence and absence of ecdysterone. The major labeled compounds found within the discs are NAD, NADP, and nicotinic acid. There is preferential uptake of nicotinamide over nicotinic acid, although the Priess-Handler pathway is used exclusively. The presence of ecdysterone produces a small increase in the NADP/NAD ratio, and an increase in NAD synthesis, probably to compensate for increased NAD turnover.
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Chambon, P., Weill, J. D., Doly, J., Strosser, M. T., and Mandel, P. (1966). On the formation of a novel adenylic compound by enzymatic extracts of liver nuclei. Biochem. Biophys. Res. Commun. 25638.
Dietrich, L. S., Fuller, L., Yero, I. L., and Martinez, L. (1966). Nicotinamide mononucleotide pyrophosphorylase activity in animal tissues. J. Biol. Chem. 241188.
Hartman, S. C., Levenburg, B., and Buchanan, J. M. (1955). Involvement of ATP, 5-phosphoribosyl-1-pyrophosphate and l-azaserine in the enzymatic formation of glycinamide ribotide intermediates in inosinic acid biosynthesis. J. Am. Chem. Soc. 77501.
Hillyard, D., Rechsteiner, M. C., and Olivera, B. M. (1973). Pyridine nucleotide metabolism in mammalian cells in culture. J. Cell Physiol. 82165.
Fristrom, J. W. (1972). The biochemistry of imaginal disc development. In Ursprung, H., and Nöthiger, R. (eds.), The Biology of Imaginal Discs, Springer-Verlag, Berlin, pp. 109–154.
Fristrom, J. W., and Mitchell, H. K. (1965). The preparative isolation of imaginal discs from larvae of Drosophila melanogaster. J. Cell Biol. 27445.
Fristrom, J. W., Raikow, R., Petri, W., and Stewart, D. (1970). In vitro evagination and RNA synthesis in imaginal discs of Drosophila melanogaster. In Hanly, E. W. (ed.), Problems in Biology: RNA in Development, University of Utah Press, Salt Lake City, pp. 381–401.
Fristrom, J. W., Gregg, T. L., and Siegel, J. G. (1974). The effect of β-ecdysone on protein synthesis in imaginal discs of Drosophila melanogaster cultured in vitro. I. The effect on total protein synthesis. Develop. Biol. 41301.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193265.
Lundquist, R., and Olivera, B. M. (1971). Pyridine nucleotide metabolism in Escherichia coli. J. Biol. Chem. 2478016.
Manlapaz-Fernandez, P., and Olivera, B. M. (1973). Pyridine nucleotide metabolism in Escherichia coli. IV. Turnover. J. Biol. Chem. 2485150.
Petri, W. H., Fristrom, J. W., Stewart, D. J., and Hanly, E. W. (1971). The in vitro synthesis and characteristics of ribosomal RNA in imaginal discs of Drosophila melanogaster. Mol. Gen. Genet. 110245.
Priess, J., and Handler, P. (1958). Enzymatic synthesis of diphosphopyridine nucleotide. J. Biol. Chem. 233493.
Rechsteiner, M., Hillyard, D., and Olivera, B. M. (1975). On the magnitude and significance of NAD turnover in the human cell line D98/AH2. Nature (in press).
Robb, J. A. (1969). Maintenance of imaginal discs of Drosophila melanogaster in chemically defined media. J. Cell Biol. 41876.
Witholt, B. (1971). A bioactographic procedure for detecting TPN, DPN, NMN, and NR. In Methods in Enzymology, Vol. XVIII, Academic Press, New York, p. 369.
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Supported by Grant GB 43569 from the National Science Foundation.
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Keyes, T.W., Olivera, B.M., Stewart, D.J. et al. Pyridine nucleotide metabolism in imaginal discs of Drosophila melanogaster . Biochem Genet 14, 197–207 (1976). https://doi.org/10.1007/BF00484760
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DOI: https://doi.org/10.1007/BF00484760