Abstract
An improved method for detecting and measuring the enzyme glucosephosphate isomerase after starch gel electrophoresis is described. Nitrocellulose filters are used in a gel overlay procedure which increases the sensitivity of the staining reaction and provides a simple means for accurate quantitation of the isozyme pattern. This staining technique may have wider application with other gel media and also with other enzymes.
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Brinster, R. L. (1973). Parental glucose phosphate isomerase activity in three-day mouse embryos. Biochem. Genet. 9187.
Carter, N.D., and Parr, C. W. (1967). Isoenzymes of phosphoglucose isomerase in mice. Nature 216511.
Carter, N. D., and Yoshida, A. (1969). Purification and characterization of human phosphoglucose isomerase. Biochim. Biophys. Acta 18112.
Champion, M. J., and Whitt, G. S. (1976). Synchronous allelic expression at the glucose phosphate isomerase A and B loci in interspecific sunfish hybrids. Biochem. Genet. 14723.
Chapman, V. M., Whitten, W. K., and Ruddle, F. H. (1971). Expression of paternal glucose phosphate isomerase-1 (Gpi-1) in preimplantation stages of mouse embryos. Dev. Biol. 26153.
DeLorenzo, R. L., and Ruddle, F. H. (1969). Genetic control of two electrophoretic variants of glucose phosphate isomerase in the mouse (Mus musculus). Biochem. Genet. 3151.
Dewey, M. J., Gervais, A. G., and Mintz, B. (1976). Brain and ganglion development from the two genotypic classes of cells in allophenic mice. Dev. Biol. 5068.
Juneja, R. K., and Wilhelmsson, M. (1975). Genetical control of phosphoglucose isomerase isozymes in the Japanese quail erythrocytes. Anim. Blood Groups Biochem. Genet. 63.
Klebe, R. J. (1975). A simple method for the quantitation of isozyme patterns. Biochem. Genet. 13805.
Markert, C. L., and Masui, Y. (1969). Lactate dehydrogenase isozymes of the penguin Pygoscelis adeliae. J. Exp. Zool. 172121.
Noltmann, E. A. (1964). Isolation of crystalline phosphoglucose isomerase from rabbit muscle. J. Biol. Chem. 2391545.
Orchardson, W. R., and McGadey, J. (1970). The histochemical demonstration of phosphoglucose isomerase. Histochemie 22136.
Peterson, A. C. (1974). Chimaera mouse study shows absence of disease in genetically dystrophic muscle. Nature 248561.
Peterson, A. C. (1977). Complete rescue of genetically dystrophic muscle in mouse chimaeras. Fed. Proc. 36555 (abst.).
Petras, M. L., Reimer, J. D., Biddle, F. G., Martin, J. E., and Linton, R. S. (1969). Studies of natural populations of Mus. V. A survey of nine loci for polymorphisms. Can. J. Genet. Cytol. 11497.
Ruddle, F. H., and Nichols, E. A. (1971). Starch gel electrophoretic phenotypes of mouse × human somatic cell hybrids and mouse isozyme polymorphisms. In Vitro 712.
Russell, E. S., Neufeld, E. F., and Higgins, C. T. (1951). Comparison of normal blood picture of young adults from 18 inbred strains of mice. Proc. Soc. Exp. Biol. Med. 78761.
Smithies, O. (1959). Zone electrophoresis in starch gels and its application to studies of serum proteins. Adv. Protein Chem. 1465.
Tsuyuki, H., Roberts, E., Kerr, R. H., and Ronald, A. P. (1966). Micro starch gel electrophoresis. J. Fish. Res. Bd. Can. 2367.
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This work was supported by the M.R.C. Group in Developmental Neurobiology, McMaster University, Canada.
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Peterson, A.C., Frair, P.M. & Wong, G.G. A technique for detection and relative quantitative analysis of glucosephosphate isomerase isozymes from nanogram tissue samples. Biochem Genet 16, 681–690 (1978). https://doi.org/10.1007/BF00484725
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DOI: https://doi.org/10.1007/BF00484725