Abstract
NAD-linked lactate, malate, glycerophosphate, alcohol and nonspecific dehydrogenases, aspartate aminotransferases, and soluble esterases from extracts of tissues of individuals from a wild population of Calomys musculinus (Rodentia, Cricetidae) have been analyzed by means of starch gel electrophoresis and specific staining. Allelic frequencies and heterozygosity have been determined. Mendelian inheritance of some of the variants detected was confirmed by breeding experiments. Ten out of fifteen (66.6%) of the genetic loci investigated presented polymorphism. Mean heterozygosity per locus was very high (H=0.2014, se 0.046).
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Blanco, A., and Gardenal, C. N. (1977). Polimorfismo enzimático en poblaciones de roedores cricétidos. Medicina) 37141 (Suppl. 3).
Blanco, A., and Zinkham, W. H. (1966). Soluble esterases in human tissues: Characterization and ontogeny. Bull. Johns Hopkins Hosp. 11827.
Blanco, A., Zinkham, W. H., and Kupchyk, L. (1964). Genetic control and ontogeny of lactate dehydrogenase in pigeon testes. J. Exp. Zool. 156137.
Bryant, E. H. (1974). On the adaptive significance of enzyme polymorphism in relation to environmental variability. Am. Nat. 1081.
Chapman, V. M., and Ruddle, F. H. (1972). Glutamate oxaloacetate transaminase (GOT) genetics in the mouse: Polymorphism of Got-1. Genetics 70299.
De Lorenzo, R. J., and Ruddle, F. H. (1970). Glutamate-oxaloacetate transaminase (GOT) genetics in Mus musculus: Linkage, polymorphism and phenotypes of the Got-2 and Got-1 loci. Biochem. Genet. 4259.
Dessauer, H. C., Nevo, E., and Chuang, K. (1975). High genetic variability in an ecologically variable vertebrate, Bufo viridis. Biochem. Genet. 13651.
de Villafañe, G. (1970). Ecología de roedores silvestres (Cricetidae) en el sur de la provincia de Córdoba, completada con observaciones sobre reproducción y desarrollo obtenidas por cría experimental. Doctoral thesis, National University of Córdoba, Córdoba.
Gardenal, C. N., Blanco, A., and Sabattini, M. S. (1977). Contribución al conocimiento de tres especies del género Calomys (Rodentia, Cricetidae): II. Análisis electroforético de formas múltiples de enzimas como criterio de diferenciación taxonómica. Physis 36179.
Gillespie, J. H., and Kojima, K. (1968). The degree of polymorphism in enzymes involved in energy production compared to that in nonspecific enzymes in two Drosophila ananassae populations. Proc. Natl. Acad. Sci. 61582.
Johnson, G. B. (1974). Enzyme polymorphism and metabolism. Science 18428.
Johnson, K. M., and Chapman, V. M. (1971). Expression of the paternal genes for glutamate-oxaloacetate transaminase (GOT) during embryogenesis in Xenopus laevis. J. Exp. Zool. 178319.
Levene, H. (1949). On a matching problem arising in genetics. Ann. Math. Stat. 2091.
Markert, C. L., and Faulhaber, I. (1965). Lactate dehydrogenase isozyme patterns of fish. J. Exp. Zool. 159319.
Nevo, E. (1976). Adaptive strategies of genetic systems in constant and varying environments. In Karlin, S., and Nevo, E. (eds.), Population Genetics and Ecology, Academic Press, New York, pp. 141–158.
Powell, J. R. (1971). Genetic polymorphism in varied environments. Science 1741035.
Powell, J. R. (1975). Protein variation in natural populations of animals. Evol. Biol. 879.
Sabattini, M. S., González de Ríos, L. E., Díaz, G., and Vega, V. R. (1977). Infección natural y experimental de roedores con virus Junin. Medicina) 37149 (Suppl. 3).
Selander, R. K. (1973). Genetic variation among vertebrate species. Annu. Rev. Ecol. Syst. 475.
Shows, T. B., Chapman, V. M., and Ruddle, F. H. (1970). Mitochondrial malate dehydrogenase and malic enzyme: Mendelian inherited electrophoretic variants in the mouse. Biochem. Genet. 4707.
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This work has been supported, in part, by grants from the Secretaria de Ciencia y Tecnología de la Nación (National Program for Endemic Diseases) and from the Fundación Emilio Ocampo. C. N. G. is a Fellow and A. B. a Career Investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas of Argentina.
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Gardenal, C.N., Sabattini, M.S. & Blanco, A. Enzyme polymorphism in a population of Calomys musculinus (Rodentia, Cricetidae). Biochem Genet 18, 563–575 (1980). https://doi.org/10.1007/BF00484402
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DOI: https://doi.org/10.1007/BF00484402