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Molecular Genetic Polymorphism of the Yeast Kluyveromyces dobzhanskii

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Abstract—

Based on strains of various ecological and geographical origins, we studied intraspecific polymorphism of the wild yeast Kluyveromyces dobzhanskii, the closest relative of the cultured dairy yeasts K. lactis and K. marxianus. Using microsatellite typing, phylogenetic analysis of the nucleotide sequences of the ITS1/ITS2 internal transcribed spacers and of the mitochondrial gene COX2, we found the species K. dobzhanskii to be of complex composition, consisting of at least three geographical populations: North American (including the type culture CBS 2104), European, and Far Eastern. Strains of different geographic origin were characterized by unique nucleotide substitutions in the ITS1/ITS2 region and in the mitochondrial COX2 gene. In European strains, a correlation was revealed between the (GTG)5 profiles and the source of isolation. The strains isolated from insects in Spain had unique patterns.

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Funding

The study was supported by a joint Russian–Taiwanese grant from the Russian Foundation for Basic Research (project no. 18-54-52002 MNT_a) and the Ministry of Science and Technology of Taiwan (MOST, project no. 107-2923-B-007-001-MY3).

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Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms, National Research Center “Kurchatov Institute”, 117519, Moscow, Russia

    E. S. Naumova & G. I. Naumov

  2. Department of Applied Science, National Tsing Hua University of Education, 30014, Hsinchu, Taiwan

    Ch.-Fu Lee

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  1. E. S. Naumova
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  2. Ch.-Fu Lee
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  3. G. I. Naumov
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Correspondence to E. S. Naumova.

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Translated by E. Babchenko

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Naumova, E.S., Lee, CF. & Naumov, G.I. Molecular Genetic Polymorphism of the Yeast Kluyveromyces dobzhanskii. Microbiology 90, 500–506 (2021). https://doi.org/10.1134/S0026261721040093

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