Abstract
The electrophoretic pattern of alcohol dehydrogenase (ADH) of Drosophilia mojavensis is composed of multiple bands. In previous studies from this laboratory we suggested on the basis of genetic evidence that multiple ADH bands were due to the presence of a gene duplication. In the studies presented here, this hypothesis is supported by data derived from comparing the protein biochemistry of each ADH. Three forms of D. mojavensis ADH have been isolated. These are the ADH-1 homodimer, the ADH-2 homodimer, and the ADH-1 ADH-2 interlocus heterodimer. Each of these isozymes has a native molecular weight of approximately 50,000. Each native molecule is composed of two subunits of identical size, 24,000 daltons. The native molecules differ slightly in their isoelectric points. Thermal denaturation also reveals that ADH-1 and ADH-2 are slightly different, ADH-1 being somewhat more thermostable. The interlocus heterodimer has properties intermediate between those of ADH-1 and those of ADH-2. Kinetic comparison also indicates a similarity among the three isozymes. ADH-2 is somewhat better at oxidizing ethanol relative to 2-propanol as compared to ADH-1. All of our studies support the general conclusion that the isozymes of ADH found in D. mojavensis are similar to one another and to other ADH from other species of Drosophila. This supports our hypothesis that multiple bands of ADH in D. mojavensis reflect the presence of a duplication of the Adh locus in that species.
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Batterham, P., Starmer, W. T., and Sullivan, D. T. (1982). Biochemical genetics of the alcohol longevity response of Drosophilia mojavensis. In Barker, J. S. F., and Starmer, W. T. (eds.), Ecological Genetics and Evolution: The Cactus-Yeast-Drosophila Model System, Academic Press, Sydney.
Batterham, P., Lovett, J. A., Starmer, W. T., and Sullivan, D. T. (1983a). Differential regulation of duplicate alcohol dehydrogenase genes in Drosophila mojavensis, Devel. Biol. 96346.
Batterham, P., Chambers, G. K., Starmer, W. T., and Sullivan, D. T. (1983b). Origin and expression of an alcohol dehydrogenase gene duplication (submitted).
Cleland, W. W. (1979). Methods in Enzymology Vol. 63 Academic Press, New York, pp. 103–108.
Fogleman, J. C. (1982). The role of volatiles in the ecology of cactophilic Drosophila. In Barker, J. S. F., and Starmer, W. T. (eds.), Ecological Genetics and Evolution: The Cactus-Yeast-Drosophila Model System, Academic Press, Sydney.
Heed, W. B. (1978). Ecology and genetics of Sonoran desert Drosophila. In Brussard, P., (ed.), Ecology and Genetics: The Interface. Springer-Verlag, New York.
Heed, W. B. (1982). The origin of Drosophila in the Sonoran Desert. In Barker, J. S. F., and Starmer, W. T. (eds.), Ecological Genetics and Evolution: The Cactus-Yeast-Drosophila Model System, Academic Press, Sydney.
Johnson, G. B. (1978). Structural flexibility of isozyme variants: Genetic variants in Drosophila disguised by cofactor and subunit binding. Proc. Natl. Acad. Sci. USA 75395.
Papel, I., Henderson, M., Van Herrewege, J., David, J., and Sofer, W. (1979). Drosophila alcohol dehydrogenase activity in vitro and in vivo: Effects of acetone feeding. Biochem. Genet. 17553.
Oakeshott, J. G., Chambers, G. K., East, P. D., Gibson, J. B., and Barker, J. S. F. (1982). Evidence for a genetic duplication involving alcohol dehydrogenase genes in Drosophila buzzatii and related species. Austr. J. Biol. Sci. 3573.
Richardson, R. H., Smouse, B. E., and Richardson, M. R. (1977). Patterns of molecular variation. II. Associations of electrophoretic mobility and larval substrate within species of the Drosophila mulleri complex. Genetics 85141.
Sofer, W., Ursprung, H. (1968). Drosophila alcohol dehydrogenase. J. Biol. Chem. 2433110.
Starmer, W. T., Heed, W. B., and Rockwood-Sluss, E. S. (1977). Extension of longevity in Drosophila mojavensis by environmental ethanol: Differences between subraces. Proc. Natl. Acad. Sci. USA 74387.
Vacek, D. (1979). Ph.D. dissertation, University of Arizona, Tucson.
Wasserman, M. (1982). Evolution of the repleta group. In Ashburner, M., Carson, H. L., and Thompson, J. N., Jr. (eds.), The Genetics and Biology of Drosophila, Vol. 3B, Academic Press, New York.
Zouros, E. (1974). Genetic differentiation associated with the early stages of speciation in the mulleri subgroup of Drosophila. Evolution 27601.
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Supported by NIH Grant AG02064.
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Batterham, P., Gritz, E., Starmer, W.T. et al. Biochemical characterization of the products of the Adh loci of Drosophila mojavensis . Biochem Genet 21, 871–883 (1983). https://doi.org/10.1007/BF00483946
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DOI: https://doi.org/10.1007/BF00483946