Skip to main content
SpringerLink
Log in

Immunology of histoplasmosis: Humoral and cellular activity from a polysacchari-de-protein complex and its deproteinized fraction in experimentally immunized mice

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

In a previous publication it was reported that a polysaccharide-protein complex (PPC), sensitive to β-glucosidase, was isolated from Histoplasma capsulatum. This complex was strongly reactive in an agar gel diffusion assay with sera from patients with histoplasmosis, but was unreactive with sera from patients with coccidioidomycosis. Here, the studies with human sera have been expanded and attempts were made to determine the response of mice immunized with nonviable H. capsulatum or Cocccidioides immitis to PPC or its deproteinized fraction (D-PPC) using more sensitive tests for antibody and including also test for cell-mediated immunity. Histoplasmin and coccidioidin were compared with PPC or its deproteinized fraction (D-PPC) in all assays. In a counterimmunoelectrophoresis (CIE) assay, PPC and D-PPC reacted only with sera from patients with histoplasmosis, whereas cross reactions were noted with histoplasmin and coccidioidin using heterologous sera. Cross-reaction were observed with all four antigen preparations and both types of antisera using a micro complement fixation assay. The assay for macrophage migration inhibitory factor (MIF) was also relatively nonspecific, in that inhibition occurred with cells from animals sensitized with Histoplasma or Coccidioides using both homologous and heterologous antigens. In the footpad assay, histoplasmin and coccidioidin were highly cross-reactive in animals sensitized with the heterologous fungus, but the PPC and D-PPC from H. capsulatum elicited significant reactions only in animals sensitized with Histoplasma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adamson, D.M. & C.G. Cozad. 1969. Effect of anti-lymphocyte serum on animals experimentally infected with Histoplasma capsulatum or Cryptococcus neoformans. J. Bact. 100: 1271–1276.

    Google Scholar 

  2. Azuma, I. et al. 1974. Chemical and immunological properties of galactomannan obtained from Histoplasma duboisii, Histoplasma capsulatum, Paracoccidioides brasiliensis and Blastomyces dermatitidis. Mycopathol. Mycol. Appl. 54: 111–126.

    Google Scholar 

  3. Biggar, W.D.; H.J. Meuwissen & R.A. Good. 1971. Successful defense against Histoplasma capsulatum in hypogammaglobulinemia. Arch. Intern. Med. 128: 585–587.

    Google Scholar 

  4. Bloom, B.R. & B. Bennett. 1971. In vitro methods of cell mediated immunity. B.R. Bloom & P. Glade, Ed. Academic Press, New York. 235–248.

    Google Scholar 

  5. Bonilla-Sotto, O.; N.R. Rose & C.E. Alberman. 1961. Allergenic Molds. Antigenic and Allergenic properties of Alternatia tenuis. J. Allergy. 31: 246–270.

    Google Scholar 

  6. Bradley, G.; L. Pine; M.W. Reeves & C.W. Moss. 1974. Purification, composition and serological characterization of histoplasmin H & M antigens. Infect. Immun. 9: 870–880.

    Google Scholar 

  7. Campbell, C.C. & G.E. Binkley. 1953. Serologic diagnosis with respect to histoplasmosis, coccidioidomycosis and blastomycosis and the problem of cross reactions. J. Lab. Clin. Med. 42: 896–906.

    Google Scholar 

  8. Campbell, C.C.; M.E. Reca & C. Ganover. 1965. Reaction of Histoplasma capsulatum antigens in sera from mammalian species including man, infected with C. immitis. Proceedings of the Second Symposium on Coccidioidomycosis 8–10 Dec., Phoenix, Arizona, 253–247.

  9. Dickerson, Q.H. Jr. et. al. 1968. Chromatographic separation of H and M antigens from histoplasmin. Proc. Soc. Exp. Biol. Med. 128: 654–658.

    Google Scholar 

  10. Domer, J.E. 1971. Monosaccharide and chitin contens of cell walls of Histoplasma capsulatum and Blastomyces dermatitidis. J. Bacteriol. 107: 870–877.

    Google Scholar 

  11. Domer, J.E.; J.G. Hamilton & J.C. Harkin. 1967. Comparative study of the cell walls of the yeast-like and mycelial phase of Histoplasma capsulatum. J. Bacteriol. 94: 466–474.

    Google Scholar 

  12. Dyson, J.E. Jr. & E.E. Evans. 1955. Delayed hypersensitivity in experimental fungus infections: The skin reactivity of antigens from yeast phase of Histoplasma capsulatum. J. Lab. Clin. Med. 45: 499–454.

    Google Scholar 

  13. Hassid, W.Z. & S. Abraham. 1957. Chemical procedure for analysis of polysaccharides. I Methods In Enzymology. SP Collowick & No. 0. Kaplan. Academic Press. New York 3: 34.

    Google Scholar 

  14. Jink, E.W. & B.G. Price. 1968. Chemical, serologic and dermal hypersensitivity activities of two fractions of histoplasmin. Amer. Rev. Resp. Dis. 98: 474–478.

    Google Scholar 

  15. Kabat. E.A. 1961. In Kabat and Mayer's, Experimental Immunochemistry. 2nd. Ed. pp. 530–561 Ed. Charles, C. Thomas, Springfild, III.

    Google Scholar 

  16. Kaufman, L. et al. 1966. Occurrence of serotypes among Histoplasma capsulatum strains. J. Bact. 91: 1438–1439.

    Google Scholar 

  17. Kirby, K.S. 1956. A new method for the isolation of ribonucleic acids from mammalian tissues. J. Biochem. 64: 405–407.

    Google Scholar 

  18. Kleger, B. & L. Kaufman. 1973. Detection and identification of diagnostic Histoplasma capsulatum precipitates by Counterelectrophoresis. Appl. Microbiol. 26: 231–238.

    Google Scholar 

  19. Lowry, O.H.; N.Y. Rosebraugh; A.L. Tarr & R.J. Randall. 1951. Protein measurements with the Folin reagent. J. Biol. Chem. 193: 265–275.

    Google Scholar 

  20. Manych, J. 1970. Critical study of positive histoplasmin, coccidioidin, blastomycin and paracoccidioidin skin allergy test. Checkoslovakia. Med. Fac. Hyg. 10: 361–372.

    Google Scholar 

  21. Marcus, S. 1970. Immunity of histoplasmosis induced in mice by components of Histoplasma capsulatum. Amer. Rev. Dis. 102: 614–622.

    Google Scholar 

  22. Markowitz, H. 1964. Polysaccharide antigens from Histoplasma capsulatum. Proc. Soc. Exp. Biol. Med. 115: 697–700.

    Google Scholar 

  23. O'Connell, E.J.; P.E. Hermans & H. Markowitz. 1967. Skin reactive antigens of histoplasmin. Exptl. Biol. Med. 124: 1015–1020.

    Google Scholar 

  24. Ouchterlony, O. 1962. Diffusion in gel methods for immunological analysis. Prog. Allergy 6: 30–154.

    Google Scholar 

  25. Public Health Service. 1965. Standardized diagnostic complement fixation method and adaptation to microtest. U.S. Public Health Serv. Publ. 1228.

  26. Reiss, E. & O. Mitchell. 1974. Cellular immune activity of a galactomann-protein complex from mycelia of Histoplasma capsulatum. Infec. Immun. 10: 802–809.

    Google Scholar 

  27. Rifkin, D. et al. 1970. Delayed hypersensitivity to fungal antigens in mice. I. Use of the intradermal skin and foodpad swelling tests as assays of active and passive sensitization. J. Infect. Dis. 133: 50–56.

    Google Scholar 

  28. Salvin, S.B. & R.F. Smith. 1959. Antigens from the yeast phase of Histoplasma capsulatum: III. Isolation properties and activity of a protein-carbohydrate complex. J. Infect. Dis. 105: 45–73.

    Google Scholar 

  29. Sevag, M.G. 1934. Eine neuve physikalische. Enteiweissungsmethode zur Darstellung biologisch. Wirksamer Substanzen. Isolierung von Kohlenhydraten aus Huhnereiweiss and Pneumococcen. Biochem. Z. 273: 419–429.

    Google Scholar 

  30. Smith, C.E.; E.C. Whiting; E.C. Baker; H.G. Rosenberger; R.R. Beard & M.T. Saito. 1948. The use of coccidioidin. Amer. Rev. Tuberc. 57: 330–360.

    Google Scholar 

  31. Sprouse, R.F.; N.L. Goodman & H.W. Larsh. 1969. Fractionation, isolation and chemical characterization of skin test active components of histoplasmin. J. Inst. Soc. Hum. Anim. Mycol. 7: 1–11.

    Google Scholar 

  32. Sweet, G.H. 1971. Antigens of Histoplasma capsulatum: II. Separation and characterization of yeast-phase precipitinogens. Amer. Rev. Resp. Dis. 104: 401–407.

    Google Scholar 

  33. Taylor, M.L. & L.F. Bojalil. 1977. Inmunologia de la histoplasmosis: Aislamiento de un complejo polisacaridoprotetína (CPP) con actividad inmuno específica a partir de Histoplasma capsulatum. Arch. Inv. Med. 8: 91–102.

    Google Scholar 

  34. Thor, D.E & S. Dray. 1968. A correlation of human delayed hypersensitivity: specific inhibition of capillary tube migration of sensitized human lymph node cells by tuberculin and histoplasmin. J. Immunol. 101: 51–61.

    Google Scholar 

  35. Tuesta, G. 1966. Biological relationships of Blastomyces, Paracoccidioides and Histoplasma. An. Fac. Med. Lima 48: 82–108.

    Google Scholar 

  36. Webb, J.M. & H.B. Levy. 1968. New developments in the chemical determination of nucleic acids. Methods in Biochemical Analysis. 6: 1–30.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Ecología, Humana. Fac. Medicina UNAM., México 20, D.F.

    M. Lucia Taylor, M. Rocío Reyes Montes, A. Lachica, C. Eslava Campos, J. Olvera & R. Maxwell

Authors
  1. M. Lucia Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Rocío Reyes Montes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Lachica
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Eslava Campos
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Olvera
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. Maxwell
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taylor, M.L., Reyes Montes, M.R., Lachica, A. et al. Immunology of histoplasmosis: Humoral and cellular activity from a polysacchari-de-protein complex and its deproteinized fraction in experimentally immunized mice. Mycopathologia 71, 159–166 (1980). https://doi.org/10.1007/BF00473064

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00473064

Keywords

Search

Navigation