Abstract
Early papers revealed that culture filtrate of Byssochlamys nivea has antibiotic properties. Active filtrate of B. nivea is extracted with ethyl acetate. Paper chromatography is carried out on Whatman no. 1; thin-layer chromatography is carried out on Kieselgel; visualization with bioautographic technique reveals a single spot.
Ethyl acetate extract is passed through silicic acid-celite column. Each isolated fraction is tested for antibiotic activity. Only one supports this activity; a crystallized matter, melting point 109–110°C, thermostable, is obtained.
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References
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Colin, AM., Percebois, G. Antibiose manifestèe par une souche de Byssochlamys nivea Westling, 1909. III. Caractèristiques chromatographiques et isolement de la fraction active. Mycopathologia 55, 5–8 (1975). https://doi.org/10.1007/BF00467082
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DOI: https://doi.org/10.1007/BF00467082