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Antimicrobial Activity of Bacteria Isolated from the Millipedes Nedyopus dawydoffiae and Orthomorpha sp.

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Abstract

From the intestines of millipedes of the species Nedyopus dawydoffiae and Orthomorpha sp. (class Diplopoda) and from their food substrates (plant residues), 72 bacterial strains belonging to 25 genera were isolated and identified. Among the studied strains, actinobacteria predominated, among which streptomycetes were the most numerous, although representatives of 14 other genera of actinobacteria were also present. High abundance of actinobacteria with antimicrobial activity was noted, including members of the “rare” genera Actinoplanes, Amycolatopsis, Kitasatospora, Lechevalieria, Micromonospora, Nocardiopsis, and Saccharopolyspora. This is the first report on antimicrobial activity in Kitasatospora saccharophila INA 01226 and Nocardiopsis umidischolae INA 01230. Heterogeneity in terms of antibiotic formation in the populations of Streptomyces pratensis and S. termitum was shown. The most promising bacterial strains chosen for the chemical study of antibiotics formed exhibited activity against methicillin-resistant Staphylococcus aureus INA 00761 (MRSA) and vancomycin-resistant strain Leuconostoc mesenteroides VKPM B-4177 (VRLM).

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ACKNOWLEDGMENTS

The authors are grateful to I.I. Semenyuk for collecting the millipedes.

Funding

The work of A.V. Yakushev on isolation of mycelial actinobacteria was conducted according to State Assignment no. 121040800174-6 “Soil Microbiomes: Genomic Diversity, Functional Activity, Geography, and Biotechnological Potential” and isolation of unicellular bacteria was financially supported by the Ministry of Science and Higher Education of the Russian Federation, project no. 075-15-2021-1396.

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Authors and Affiliations

  1. Gause Institute of New Antibiotics, 119021, Moscow, Russia

    T. A. Efimenko, A. A. Karabanova, A. A. Glukhova, M. V. Demiankova, B. F. Vasilieva, Yu. V. Boykova, N. D. Malkina, L. P. Terekhova & O. V. Efremenkova

  2. Faculty of Soil Science, Moscow State University, 119991, Moscow, Russia

    A. V. Yakushev

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  1. T. A. Efimenko
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  2. A. V. Yakushev
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  4. A. A. Glukhova
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  5. M. V. Demiankova
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  8. N. D. Malkina
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Correspondence to T. A. Efimenko.

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Translated by D. Timchenko

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Efimenko, T.A., Yakushev, A.V., Karabanova, A.A. et al. Antimicrobial Activity of Bacteria Isolated from the Millipedes Nedyopus dawydoffiae and Orthomorpha sp.. Microbiology 91, 770–782 (2022). https://doi.org/10.1134/S0026261722700023

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