Summary
Using a previously described experimental model for demonstration of osteoinduction biological activity of variably prepared fractions of demineralized bone matrix was studied. Sequential extraction of matrix using CaCl2, EDTA, LiCl and water results in an acceleration of the induction process and a reduction of immunological host reaction in the case of xenogenic implants. Application of chaotropic reagents such as guanidinium chloride and sodium thiocyanate has the consequency of lowering the inductive signal. From these results it is likely that noncollagenous constituents of bone matrix play a significant role in osteoinduction.
Zusammenfassung
Anhand eines schon früher beschriebenen Versuchsmodells (Teil I) zum Nachweis der Osteoinduktion durch demineralisierte Matrix werden Eigenschaften unterschiedlich bearbeiteter Matrixpräparationen aufgezeigt. Dabei findet sich nach einer schrittweisen Extraktion der Matrix mit Calciumchlorid, EDTA, Lithiumchlorid und Wasser neben einer Steigerung und Beschleunigung des ausgelösten Induktionsvorganges im Empfänger eine verminderte immunologische Abwehrreaktion bei xenogener OCG. Die Anwendung chaotrop wirkender Extraktionsmittel wie Guanidiniumchlorid und Natriumthiocyanat führt zu einer Auslöschung bzw. Minderung des osteoinduktiven Signals. Aus diesen Untersuchungen kann auf die Bedeutung der nicht kollagenen Bestandteile der Matrix für die Osteoinduktion geschlossen werden.
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This work was supported by Deutsche Forschungsgemeinschaft Ve 56/5-1
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Thielemann, F.W., Schmidt, K. & Koslowski, L. Osteoinduction. Arch. Orth. Traum. Surg. 100, 73–78 (1982). https://doi.org/10.1007/BF00462342
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DOI: https://doi.org/10.1007/BF00462342