Über das Schicksal der Matrix von konservierten Knorpel-Mittelohrtransplantaten

Summary

By means of autoradiography preserved cartilage homografts are studied after transplantation into the bullas of rabbits. The donor animal had received tritiated aminoacids, phenylaline and tyrosine on seven consecutive days. Autoradiographs of the cartilage of the donor animal showed a uniform distribution of silvergrains in the emulsion convering the histologic slide indicating the H³-aminoacids incorporated into the proteins of the mucopolysaccharide protein complex. During the time of observation no change in the silvergrainpattern was observed except in areas of resorption where a remarcable decrease of radioactivity can be found. The activity concentrates over connective tissue cells resorbing small parts of the transplant. This observation indicates that the cells play an active part in the brake down of the matrical proteins. The labelled fragments pass an intracellular stage before the majority is removed by the body fluids. A small quantity may be reused, if bone formation occurrs. As shown by other authors for the formation of the cartilage matrix, this study proves a transcellular pathway of matrical constituents for the resorbtion as well.

Zusammenfassung

Es wird über experimentelle Untersuchungen über das Schicksal von Knorpeltransplantaten in der Bulla des Kaninchens berichtet. Die Transplantate waren vor der Entnahme aus dem Spendertier durch H³-Tyrosin und H³-Phenylalanin radioaktiv markiert worden. Mit Hilfe der Autoradiographischen Nachweismethode konnte gezeigt werden, daß sich die Radioaktivität nur im Bereich von einwachsendem Bindegewebe verändert. Ein Abbau der Intercellularsubstanz allein durch die Gewebsflüssigkeiten des Wirtsorganismus erfolgt nicht.