Summary
The cochlea is quick-frozen and then opened while under liquid nitrogen to expose the scalae of all turns in the mid-modiolar section. Still under liquid nitrogen, the cochlea is transferred to the cold stage of a pre-vacuum chamber especially constructed for attachment to the scanning electron microscope. Under partial vacuum, it is moved to the cold stage in the high vacuum chamber of a field-emission scanning electron microscope. Energy dispersive X-ray analysis demonstrates the relative levels of sodium, potassium, and chlorine in the fluid spaces of the cochlea.
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References
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This work was supported by Defence and Civil Institute of Environmental Medicine, Research Contracts nos. 3277052/8SU78-00037 and 3279011/8SU79-00171. DCIEM Research Paper 80P35
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Hunter-Duvar, I., Landolt, J. & Cameron, R. X-ray microanalysis of fluid spaces in the frozen cochlea. Arch Otorhinolaryngol 230, 245–249 (1981). https://doi.org/10.1007/BF00456325
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DOI: https://doi.org/10.1007/BF00456325