Abstract
The concentration of adenosine tri-, adenosine di-, and adenosine monophosphate in cells of Acanthamoeba castellanii was measured during the logarithmic growth phase and the stationary growth phase in which trophozoites were transformed into cysts. This developmental process was induced in three ways: by growth in nutrient medium to high cell density, by transferring cells in the logarithmic phase into a nutrient-free medium, and by mixing logarithmically growing cells with ethidium bromide.
In all cases, encystment is accompanied with a reduction of total adenosine phosphate content to about 85%, mainly because of a depletion of cellular ATP. The value of the adenosine phosphate energy charge in logarithmically growing amoebae is 0.83. During development the energy charge becomes stabilized at different values (between 0.58 and 0.81), characteristic to the mode of encystation.
A possible functional relationship between changes of the adenosine phosphate concentration and developmental processes of the amoeba is discussed.
Zusammenfassung
Die Konzentration von Adenosintri-, Adenosindi- und Adenosinmonophosphat wurde in Acanthamoeba castellanii währed der logarithmischen Wachstumsphase und der stationären Wachstumsphase, in deren Verlauf sich Trophozoiten zu Cysten entwickeln, bestimmt. Der Entwicklungsprozess wurde durch drei verschiedene Methoden hervorgerufen: Durch Wachstum im Nährmedium zu großer Zelldichte, durch Überführen von Amöben der logarithmischen Wachstumsphase in ein nährstoffreies Salzmedium und durch Versetzen logarithmisch wachsender Amöben mit Äthidiumbromid.
In allen Fällen wird der Adenosinphosphat-Gehalt in den Zellen im Laufe der Entwicklung um etwa 85% reduziert, wozu besonders die Abnahme des ATP-Gehaltes beiträgt. Die Adenosinphosphat-“energy charge” beträgt in logarithmisch wachsenden Amöben 0,83. Im Laufe der Entwicklung wird sie je nach Encystierungsbedingungen auf unterschiedlichen Werten stabilisiert (zwischen 0,58 und 0,81). Die Möglichkeit eines Zusammenhanges von Konzentrationsveränderungen der Adenosinphosphate und entwicklungsspezifischen Prozessen wird diskutiert.
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Jantzen, H. Das Adenosinphosphat-System während Wachstum und Entwicklung von Acanthamoeba castellanii . Arch. Microbiol. 101, 391–399 (1974). https://doi.org/10.1007/BF00455955
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DOI: https://doi.org/10.1007/BF00455955