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Inhibition of nitrate uptake by ammonia in a blue-green alga, Anabaena cylindrica

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Abstract

Ammonia at concentrations above 1×10-5 M inhibits uptake of nitrate in the nitrogen-fixing blue-green alga, Anabaena cylindrica. This inhibition takes place both in the light and in the dark. The rate of nitrate uptake is stimulated by light. Addition of relatively high concentrations of nitrate (1–10 mM) reversibly inhibits ammonia uptake. FCCP, an uncoupler of phosphorylation, inhibits both nitrate and ammonia uptake. Ammonia may inhibit nitrate uptake by reducing the supply of energy (ATP) for active nitrate transport.

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Abbreviations

FCCP:

carbonyl cyanide p-trifluoromethoxy-phenylhydrazone

CCCP:

carbonyl cyanide m-chlorophenyl-hydrazone

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Ohmori, M., Ohmori, K. & Strotmann, H. Inhibition of nitrate uptake by ammonia in a blue-green alga, Anabaena cylindrica . Arch. Microbiol. 114, 225–229 (1977). https://doi.org/10.1007/BF00446866

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