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Determination of the isotope distribution in malate-14C with fumarase-negative lactic acid bacteria

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Abstract

No fumarase activity could be found in whole cells or in cell-free crude extracts from Leuconostoc mesenteroides or Lactobacillus curvatus. The degradation of l-malate-4-14C by these organisms yielded more than 95% of the label as 14CO2. It is therefore recommended that these organisms, rather than Lactobacillus plantarum, should be used in the determination of isotope distribution in l-malate-14C, since L. plantarum exhibits a significant fumarase activity and thus randomizes malate prior to the decarboxylation of this substance by the malolactic enzyme.

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  1. Botanisches Institut der Universität München, Menzinger Str. 67, D-8000, München 19, Germany

    J. Winter & O. Kandler

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  1. J. Winter
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  2. O. Kandler
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Winter, J., Kandler, O. Determination of the isotope distribution in malate-14C with fumarase-negative lactic acid bacteria. Arch. Microbiol. 112, 99–102 (1977). https://doi.org/10.1007/BF00446660

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  • DOI: https://doi.org/10.1007/BF00446660

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