Abstract
P/2e ratios were calculated from anaerobic chemostat cultures of Paracoccus denitrificans with nitrogenous oxides as electron acceptor. P/2e ratios were calculated, using the Y maxATP values determined for aerobic cultures. When succinate was the carbon and energy source the average P/2e values of the sulphate-and succinate-limited cultures with nitrate as electron acceptor were 0.5 and 0.7, respectively, and of the nitrite-limited culture 0.9. With gluconate as carbon and energy source the average P/2e values of the gluconate-limited with nitrate as electron acceptor and nitrate limited cultures were 0.9 and 1.1, respectively.
→H+/O ratios measured in cells obtained from sulphate-, succinate, nitrite-, gluconate-and nitratelimited cultures yielded respective average values of 3.4, 4.5, 3.5, 4.8 and 6.2 for endogenous substrates. From our data we conclude that sulphate-and nitritelimitation causes the loss of site I phosphorylation. Nitrite has no influence on the maximum growth yield on ATP. We propose that metabolism in heterotrophically grown cells of Paracoccus dentrificans is regulated on the level of phosphorylation in the site I region of the electron transport chain.
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Boender R. J., Stouthamer, A. H.: A method for the determination of confidence limits for the P/2e ratio for chosen values of Y maxATP from the results of continuous culture experiments. Arch. Microbiol. (in press, 1977)
Chang, J. P., Morris, J. G.: Studies on the utilisation of nitrate by Micrococcus denitrificans. J. gen. Microbiol. 29, 301–310 (1962)
Davis, D. H., Doudoroff, M., Stanier, R. Y.: Proposal to reject the genus hydrogenomas: taxonomic implications. Int. J. Syst. Bact. 19, 375–390 (1969)
Ferguson, S. J., John, P., Lloyd, W. J., Radda, G. K., Whatley, F. R.: The ATP-ase as an irreversible component in electron transport linked ATP synthesis. FEBS Letters 62, 272–275 (1976)
Forget, P.: Recherches sur le métabolisme du glucose chez les bactéries denitrifiantes II. Micrococcus denitrificans. Ann. Inst. Pasteur 115, 332–342 (1968)
Geary, R. C.: The frequency distribution of the quotient of two normal variaties. J. roy. Statist. Soc. 93, 442–446 (1930)
van Gent-Ruijters, M. L. W., de Vries, W., Stouthamer A. H.: Influence of nitrate on fermentation pattern, molar growth yields and synthesis of cytochrome b in Propionibacterium pentosaceum. J. gen. Microbiol. 88, 36–48 (1975)
Hadjipetrou, L. P., Stouthamer, A. H.: Energy production during nitrate respiration by Aerobacter aerogenes. J. gen. Microbiol. 38, 29–34 (1965)
Hestrin, S.: Reaction of acetylcholine and other carboxylic acid derivates with hydroxylamine and its analytical application. J. biol. Chem. 180, 249–261 (1949)
Holdeman, L. V., Moore, W. E. C.: Anaerobic laboratory manual, p. 113. Virginia Polytechnic Institute and State University, Blacksburg, Virginia (1972)
Imai, K., Asano, A., Sato, R.: Oxidative phosphorylation in Micrococcus denitrificans. I Preparation and properties of phosphorylating membrane fragments. Biochim. biophys. Acta (Amst.) 143, 462–476 (1967)
Ishimoto, M., Umeyana, M., Chiba, S.: Alteration of fermentation products from butyrate to acetate by nitrate reduction in Clostridium perfringens. Z. allg. Mikrobiol. 14, 115–121 (1974)
John, P., Hamilton, W. A.: Respiratory control in membrane particles from Micrococcus denitrificans. FEBS Letters 10, 246–248 (1970)
John, P., Whatley, F. R.: Oxidative phosphorylation coupled to oxygen uptake and nitrate reduction in Micrococcus denitrificans. Biochim. biophys. Acta (Amst.) 216, 342–352 (1970)
John, P., Whatley, F. R.: Paracoccus denitrificans and the evolutionary origin of the mitochondrion. Nature (Lond.) 254, 495–498 (1975)
Kluyver, A. J.: Some aspects of nitrate reduction. 6th Intern. Congr. Microbiology, Italy, vol. 3, pp. 71–91 (1953)
Knobloch, K., Ishaque, M., Aleem, M. I. H: Oxidative phosphorylation in Micrococcus denitrificans under autotrophic growth conditions. Arch. Mikrobiol. 76, 114–124 (1971)
Koike, I., Hattori, A.: Growth yield of a denitrifying bacterium Pseudomonas denitrificans under aerobic and denitrifying conditions. J. gen. Microbiol. 88, 1–10 (1975a)
Koike, I., Hattori, A.: Energy yield of denitrification: An estimate from growth yield in continuous cultures of Pseudomonas denitrificans under nitrate, nitrite and nitrous oxide limited conditions. J. gen. Microbiol. 88, 11–19 (1975b)
de Kwaadsteniet, J. W., Jager, J. C., Stouthamer, A. H.: A quantitative description of heterotrophic growth in microorganisms. J. theor. Biol. 57, 103–120 (1976)
Lam, Y., Nicholas D. J. D.: Aerobic and anaerobic respiration in Micrococcus denitrificans. Biochim. biophys. Acta (Amst.) 172, 450–461 (1969)
Meyer, D. J., Jones, C. W.: Oxidative phosphorylation in bacteria which contain different cytochrome oxidase. Europ. J. Biochem. 36, 144–151 (1973)
Meijer, E. M., van Verseveld, H. W., van der Beek, E. G., Stouthamer, A. H.: Energy conservation during aerobic growth in Paracoccus denitrificans. Arch. Microbiol. 112, 25–34 (1977)
Newton, N.: The two heam nitrite reductase of Micrococcus denitrificans. Biochim. biophys. Acta (Amst.) 185, 316–331 (1969)
Neijssel, O. M., Tempest, D. W.: The regulation of carbohydrate metabolism in Klebsiella aerogenes NCTC 418 organisms, growing in chemostat experiments. Arch. Microbiol. 106, 251–258 (1975)
Neijssel, O. M., Tempest, D. W.: Bioenergetic aspects of aerobic growth of Klebsiella aerogenes NCTC418 in carbon limited and carbon sulficient chemostat cultures. Arch. Microbiol. 107, 215–221 (1976)
Pichinoty, F.: La reduction bactérienne des composés oxygénés minéraux de l'azote. Bull. Inst. Pasteur 71, 317–395 (1973)
Pichinoty, F., Mottet, F., Bigliardi-Rouvier, J., Forget, P.: Le cycle de Krebs chez les bactéries denitrifiantes. Z. Naturforsch. 18b, 492–494 (1963)
Pirt, S. J.: The maintenance energy of bacteria in growing cultures. Proc. roy. Soc. B 163, 224–231 (1965)
van't Riet, J., Stouthamer, A. H., Planta, R. J.: Regulation of nitrate assimilation and nitrate respiration in Aerobacter aerogenes. J. Bact. 96, 1455–1464 (1968)
Scholes, P., Smith, L.: Composition and properties of the membrane bound respiratory chain of Micrococcus denitrificans. Biochim. biophys. Acta (Amst.) 153, 363–375 (1968)
Stouthamer, A. H.: A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie v. Leeuwenhoek 39, 545–565 (1973)
Stouthamer, A. H.: Biochemistry and genetics of nitrate reductase in bacteria. Advanc. Microbiol. Physiol. 14, 315–375 (1976a)
Stouthamer, A. H.: Yield studies in microorganisms. Durham, England: Meadowfield Press 1976b
Stouthamer, A. H., Bettenhaussen, C. W.: Influence of hydrogen acceptors on growth and energy production of Proteus mirabilis. Antonie. v. Leeuwenhoek 38, 81–90 (1972)
Stouthamer, A. H., Bettenhaussen, C. W.: Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. Biochim. biophys. Acta (Amst.) 301, 53–70 (1973)
Stouthamer, A. H., Bettenhaussen, C. W.: Determination of the efficiency of oxidative phosphorylation in continuous cultures of Aerobacter aerogenes. Arch. Microbiol. 102, 187–192 (1975)
van Verseveld, H. W., Stouthamer, A. H.: Oxidative phosphorylation in Micrococcus denitrificans. Calculation of the P/O ratio in growing cells. Arch. Microbiol. 107, 241–247 (1976)
de Vries, W., Stouthamer, A. H.: Fermentation of glucose, lactose galactose, mannitol and xylose by Bifidobacteria. J. Bact. 96, 472–478 (1968)
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Van Verseveld, H.W., Meijer, E.M. & Stouthamer, A.H. Energy conservation during nitrate respiration in Paracoccus denitrificans . Arch. Microbiol. 112, 17–23 (1977). https://doi.org/10.1007/BF00446649
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DOI: https://doi.org/10.1007/BF00446649