Abstract
A gene bank of the nutritionally versatile, nitrogen-fixing cyanobacterium Chlorogloeopsis fritschii was constructed in λ Charon 4A. 2,800 recombinants containing 10–20 kbp C. fritschii DNA fragments were screened by Southern hybridization using probes containing the genes for the large (LSU) and small (SSU) subunits of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) from Anacystis nidulans. A single recombinant plaque (λCDG1) containing a 10.9 kbp EcoR1 fragment from C. fritschii hybridized to both the LSU and SSU probes, indicating a possible linkage of these RuBisCO genes in C. fritschii. RuBisCO activity and protein were detected in λCDG1 lysates of Escherichia coli. Hybridization was also obtained between C. fritschii DNA and the LSU probe from Chlamydomonas reinhardtii, although no homology was detected using the LSU probe from maize or the SSU probe from pea.
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Abbreviations
- RuBisCO:
-
d-ribulose 1,5-bisphosphate carboxylase/oxygenase
- RuBP:
-
d-ribulose 1,5-bisphosphate
- LSU:
-
large subunit of RuBisCO
- SSU:
-
small subunit of RuBisCO
- SDS:
-
sodium dodecyl sulphate
- DOC:
-
deoxycholate
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Vakeria, D., Codd, G.A., Hawthornthwaite, A.M. et al. Construction of a gene bank and identification of the ribulose bisphosphate carboxylase/oxygenase genes from the nutritionally versatile cyanobacterium Chlorogloeopsis fritschii . Arch. Microbiol. 145, 228–233 (1986). https://doi.org/10.1007/BF00443650
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DOI: https://doi.org/10.1007/BF00443650