Skip to main content
SpringerLink
Log in

Drugs and depression of lymphocyte blastogenesis in children

Trials with Phenilbutazone, Thiamphenicol, Sulfamethopyrazine, Diazepam and the antiblastic platinum diammino dichloride

  • Published:
Zeitschrift für Kinderheilkunde Aims and scope Submit manuscript

Abstract

Data are presented which demonstrate an inhibitory effect of non anti-blastic drugs with different therapeutical indications: Phenilbutazone (Ph), Thiamphenicol (Th) and Sulfamethopyrazine (Sl) on PHA and mixed stimulation of normal children peripheral blood lymphocytes, when added in vitro. Similarly a depressed response to PHA was observed for the lymphocytes of patients treated with the same drugs. When tested on the same experimental system, Diazepam (Dz) failed to show a significant inhibition.

A first trial with a new antiblastic (cis-platinum II diammino dichloride) has also been performed. The “in vitro” addition of this antiblastic strongly depressed lymphocyte blastogenesis.

The viability Trypan-Blue Exclusion Test was performed for all the drugs with inhibiting properties. No citotoxic effect could be seen when the 50% PHA-blastogenesis inhibiting dose was employed.

Zusammenfassung

Zweck dieser Arbeit war, die Einwirkung einiger grundsätzlich verschiedener Arzneimittel (Phenilbutazon: Ph, Thiamphenicol: Th, Sulfamethopyrazine: Sl, Diazepam: Dz und des Antiblasticum Cis-Platinum II diammino dichloride: PDD) auf die Blastogenese der Lymphocyten zu prüfen. Es wurde a) der Effekt der Zugabe der einzelnen Mittel zur PHA-Kultur von Lymphocyten normaler Kinder ausgewertet und b) die PHA-Blastogenese der Lymphocyten von Kindern, die sich in Behandlung mit den verschiedenen Arzneien (mit der Ausnahme vom PDD) betrafen, erforscht. Ph, Th, Sl und PDD (dieses in den in vitro-Erfahrungen) haben eine deutliche Hemmung auf die Blastogenese ausgeübt. Dz übt keine (oder nur eine sehr spärliche) Blastogenese-Depression aus. c) Wenn man dieselben Arzneimittel dem MLC-System zufügt, kommen ähnliche Ergebnisse zum Vorschein.

Bei der Anwendung im Tripan-Blau-Test von Arzneimengen, die fähig sind, die PHA-Blastogenesewerte auf 50% der Kontrollwerte herabzusetzen, haben weder das Ph, das Th und das Sl noch das PDD eine cytotoxische Wirkung erwiesen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Astaldi, A., Jr., Curtoni, E., Ugazio, A., Mingrat, G.: Comparison of the adriamycin and L-asparaginase effects on human lymphocytes in cell-culture. Blut 23, 367 (1971).

    Google Scholar 

  2. Astaldi, G., Burgio, G. R., Krc, I., Genova, R., Astaldi, A., Jr.: L-asparaginase and blastogenesis. Lancet 1969 I, 423.

  3. Astaldi, G., Lisiewicz, J.: Lymphocytes, structure, production, functions, p. 223. Naples: Idelson Publ. 1971.

    Google Scholar 

  4. Burgio, G. R., Astaldi, A., Jr., Vaccaro, R., Gasparoni, M. C.: Über die immunsuppressiven Eigenschaften der L-Asparaginase. Einwirkung auf die Lymphocyten-Mischkulturen (in vitro) und auf die Immunglobulinwerte des Serums. Mschr. Kinderheilk. 119, 439 (1971).

    Google Scholar 

  5. Burgio, G. R.: Autoimmunität-Autoimmunkrankheiten, ein Grundriß für den Pädiater. Pädiat. FortbildK. Praxis, Vol. 35, p. 127. Basel: Karger 1972.

    Google Scholar 

  6. Hagen, C., Froland, A.: Depressed lymphocyte response to PHA in women taking oral contraceptives. Lancet 1972 I, 1185.

  7. Hirschhorn, Grossman, Weissman: Quoted by [3].

  8. Marolt-Gomiscek, M.: Rifampicin: an immunosuppressant? Lancet 1971 II, 930.

  9. McElvain, T. J., Hayward, S. K.: L-Asparaginase and blastogenesis. Lancet 1969 I, 527.

  10. Müller-Ruchholtz, W.: Beeinflussung immunologischer Reaktivität durch sog. Immunosuppressiva. Mschr. Kinderheilk. 120, 206 (1972).

    Google Scholar 

  11. Nilsson, B. S.: Rifampicin: an immunosuppressant? Lancet 1971 II, 374.

  12. Schneider, W., Pappas, A., Scheurlen, P. G.: The in vitro influence of aspirin on metabolism and transformation of human blood lymphocytes. 1st Meet. Europ. Div. Int. Soc. Haematol. Milan, 1971, Abstr. p. 207.

  13. Schneider, W., Scheurlen, P. G., Pappas, A.: Investigations on the dependence of lymphocyte transformation from hexokinase reaction. 14th Intern. Congr. Haematol. Sao Paulo, Brazil, 1972.

  14. Sorrel Tania, C., Forbes, I. J., Burness, F. R., Rischbieth, R. H. C.: Depression of immunological function in patients treated with phenytoin sodium (sodium-diphenylhydantoin). Lancet 1971 II, 1233.

  15. Wagner, H. P.: Immunosuppressive Therapie. Pädiat. FortbildK. Praxis, Vol. 35, p. 156. Basel: Karger 1972.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pediatric Clinic, University Medical School of Pavia, Italy

    G. R. Burgio, A. Astaldi Jr., A. Ugazio, G. Biscatti & A. Marchi

Authors
  1. G. R. Burgio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Astaldi Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Ugazio
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Biscatti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Marchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Paper delivered at the 3rd ESPHI Scientific Meeting, Hamburg, September 9/10, 1972.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Burgio, G.R., Astaldi, A., Ugazio, A. et al. Drugs and depression of lymphocyte blastogenesis in children. Z. Kinder-Heilk. 115, 131–139 (1973). https://doi.org/10.1007/BF00440538

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00440538

Key words

Search

Navigation